Gene Expression Data
Assay Details
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Assay
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Reference:
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J:284147
Velecela V, et al., Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4. Development. 2019 Oct 17;146(20):dev178723
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6401392
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Gene symbol:
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Wt1
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Gene name:
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WT1 transcription factor
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Reporter:
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GFP
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Antibody:
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anti-GFP
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
S1 - Wt1GFP:
embryonic day 11.5; Wt1tm1Nhsn/Wt1 +
(more  )
Note: Double labeled: green - Wt1 reporter; red - Wt1 protein (assay MGI:6401394); also stained with Hoechst (blue).
Specimen
S1 - Wt1:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Wt1tm1Nhsn/Wt1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Wt1 reporter; red - Wt1 protein (assay MGI:6401394); also stained with Hoechst (blue).
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Analysis Tools
