Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:56117
Noakes PG, et al., Expression and localisation of dynamin and syntaxin during neural development and neuromuscular synapse formation. J Comp Neurol. 1999 Aug 9;410(4):531-40
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6401343
|
|
Gene symbol:
|
Sv2a
|
|
Gene name:
|
synaptic vesicle glycoprotein 2a
|
|
Antibody:
|
anti-SV2
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
The specificity of the antibody used was not detailed; all family members have been annotated.
|
|
|
Results
|
Specimen
6D:
embryonic day 14.5
(more  )
Note: Summarized in Table 1.
Specimen
6D:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Summarized in Table 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal muscle |
Present |
Regionally restricted |
6D
|
Expressed in developing neuromuscular synapses (61% colocalised with alpha-bungarotoxin).
|
| TS22: intercostal nerve |
Present |
Regionally restricted |
6D
|
Expressed in developing neuromuscular synapses (61% colocalised with alpha-bungarotoxin).
|
Specimen
not shown:
embryonic day 14.0
(more )
Note: Summarized in Table 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Summarized in Table 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: intercostal muscle |
Present |
Regionally restricted |
|
Only occasionally expressed in developing neuromuscular synapses (3% colocalised with alpha-bungarotoxin).
|
| TS22: intercostal nerve |
Present |
Regionally restricted |
|
Only occasionally expressed in developing neuromuscular synapses (3% colocalised with alpha-bungarotoxin).
|
Specimen
not shown:
embryonic day 15.5
(more )
Note: Summarized in Table 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Summarized in Table 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: intercostal muscle |
Present |
Regionally restricted |
|
Expressed in developing neuromuscular synapses (61% colocalised with alpha-bungarotoxin).
|
| TS23: intercostal nerve |
Present |
Regionally restricted |
|
Expressed in developing neuromuscular synapses (61% colocalised with alpha-bungarotoxin).
|
Specimen
not shown:
embryonic day 17.5
(more )
Note: Summarized in Table 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Summarized in Table 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: intercostal muscle |
Present |
Regionally restricted |
|
Expressed in developing neuromuscular synapses (98% colocalised with alpha-bungarotoxin).
|
| TS25: intercostal nerve |
Present |
Regionally restricted |
|
Expressed in developing neuromuscular synapses (98% colocalised with alpha-bungarotoxin).
|
Specimen
not shown:
postnatal adult
(more )
Note: Summarized in Table 1.
Specimen
not shown:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Summarized in Table 1.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: intercostal muscle |
Present |
Regionally restricted |
|
Expressed in neuromuscular synapses (93% colocalised with alpha-bungarotoxin).
|
| TS28: intercostal nerve |
Present |
Regionally restricted |
|
Expressed in neuromuscular synapses (93% colocalised with alpha-bungarotoxin).
|
|
Analysis Tools