Probe:	Dkk1 probe7
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS16: nephric duct	Present	Regionally restricted	2A	Expression detected bilaterally to cloaca. Two discrete domains corresponding to where Wolffian ducts insert into cloaca.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Present	Regionally restricted	2B	Expression near sites where Wolffian ducts insert into cloaca.
TS17: cloaca associated mesenchyme	Present	Regionally restricted	2B	Expression in dorsal (dPCM) and ventral (vPCM) but not in intermediate (iPCM) pericloacal mesenchyme.

Structure	Level	Pattern	Image	Note
TS18: genital swelling	Absent		2C	Expression undetectable when genital tubercles were apparent.
TS18: cloaca associated mesenchyme	Present	Regionally restricted	2C	Expression in dorsal (dPCM) and ventral (vPCM) but not in intermediate (iPCM) pericloacal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: cloaca associated mesenchyme	Strong	Regionally restricted	2D	Highest expression at E11.5, when intracloacal mesenchyme (ICM), dorsal pericloacal mesenchyme (dPCM) and dorsal cloacal mesenchyme (CM) is observed.
TS19: genital tubercle	Present	Regionally restricted	2D	Expression in genital tubercle, particularly in dorsal and distal region beginning at E11.5.

Structure	Level	Pattern	Image	Note
TS19: cloaca associated mesenchyme	Strong	Regionally restricted	2D inset	Expression in dorsal (dPCM) and ventral (vPCM) pericloacal mesenchyme.
TS19: limb bud	Present		2D inset

Structure	Level	Pattern	Image	Note
TS20: cloaca associated mesenchyme	Trace	Regionally restricted	2E	Significantly diminished expression in dorsal pericloacal mesenchyme (dPCM). Expression undetectable in ventral pericloacal mesenchyme (vPCM).
TS20: genital tubercle	Present	Regionally restricted	2E	Expression in genital tubercle, particularly in dorsal and distal region, persisted at E12.5.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle	Present	Regionally restricted	2F	Expression in genital tubercle, particularly in dorsal and distal region, persisted at E13.5.

Structure	Level	Pattern	Image	Note
TS17: cloaca associated mesenchyme	Present	Regionally restricted	2G	Expression in dorsal pericloacal mesenchyme (dPCM).

Structure	Level	Pattern	Image	Note
TS19: cloaca associated mesenchyme	Present	Regionally restricted	2H	Expression in dorsal (dPCM) and ventral (vPCM) but not in intermediate (iPCM) pericloacal mesenchyme.
TS19: genital tubercle	Present	Regionally restricted	2H	Expression in both ectodermal epithelium and mesenchymal cells immediately underlying surface ectoderm of genital tubercle.

Structure	Level	Pattern	Image	Note
TS20: cloaca associated mesenchyme	Present	Regionally restricted	2I	Expression in ventral (vPCM) but not in intermediate (iPCM) pericloacal mesenchyme .

Structure	Level	Pattern	Image	Note
TS21: genital tubercle epithelium	Present		2J
TS21: genitourinary system epithelium	Present	Regionally restricted	2J	No expression in cloacal and urethral endodermal epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle epithelium	Present		2K	Expression in both ectodermal epithelium of genital tubercle and in mesenchymal cells immediately underlying surface ectoderm of genital tubercle.
TS21: genital tubercle mesenchyme	Present		2K	Expression in both ectodermal epithelium of genital tubercle and in mesenchymal cells immediately underlying surface ectoderm of genital tubercle.
TS21: genitourinary system epithelium	Present	Regionally restricted	2K	No expression in cloacal and urethral endodermal epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle mesenchyme	Present		2L	Expression in mesenchymal cells immediately underlying surface ectoderm of genital tubercle.
TS21: genitourinary system epithelium	Present	Regionally restricted	2L	No expression in cloacal and urethral endodermal epithelial cells.