Antibody:	AQP4 (sc-20812) antibody
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS24: basal forebrain	Present	Regionally restricted	2a	Expressed in the ventral region under the pial surface, and on few cell processes.

Structure	Level	Pattern	Image	Note
TS25: basal forebrain	Present	Regionally restricted	2b	Dramatically increased expression in few processes close to the pia mater. No glial processes contacting blood vessels stained positive at this stage.

Structure	Level	Pattern	Image	Note
TS26: basal forebrain	Strong	Regionally restricted	2c	Dramatically increased expression in cell processes.

Structure	Level	Pattern	Image	Note
TS27: basal forebrain	Present		2d	Expression was increasingly restricted to subpial and perivascular endfeet as is typically found in the adult brain.

Structure	Level	Pattern	Image	Note
TS27: basal forebrain	Present		2e	Expression was increasingly restricted to subpial and perivascular endfeet as is typically found in the adult brain.

Structure	Level	Pattern	Image	Note
TS27: basal forebrain	Present		2f	Expression was increasingly restricted to subpial and perivascular endfeet as is typically found in the adult brain.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Trace		3a	Scarce if any expression.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Trace		3b	Little expression in perivascular area.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		3c	Expressed in perivascular area, subpial and radial cell processes.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		3d	Expressed in subpial and radial cell processes.
TS27: brain blood vessel	Present		3d

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		3e	Expressed in subpial and radial cell processes.
TS27: brain blood vessel	Present		3e

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		3f	Expressed in subpial and radial cell processes, and astrocytic processes.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	4a	No expression in this dorsal cortex. Mainly expressed in the ventral region (see Fig 2a).

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Trace	Regionally restricted	4b	Little expression in perivascular area.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		4c	Expressed in radial processes. Delayed staining pattern in this dorsal region.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		4d	Expressed in radial processes more clearly.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		4e	Expressed in radial processes.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		4f	Expressed in radial processes and astrocytic star shaped processes.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present		5a middle , 5a right	But these positive stained fibers were not positive for GFAP.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		5b right , 5b middle	Double labeled radial fibers were clearly seen.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present		5c middle , 5c right	Double labeled star-shaped astrocytes appeared.

Structure	Level	Pattern	Image	Note
TS23: nasal cavity mucosa	Present	Regionally restricted	S1	Expressed in olfactory mucosa.
TS23: brain	Absent		S1