Gene Expression Data
Assay Details
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Assay
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Reference:
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J:280430
Janeckova E, et al., Dynamic activation of Wnt, Fgf, and Hh signaling during soft palate development. PLoS One. 2019;14(10):e0223879
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6389042
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Gene symbol:
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Axin2
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Gene name:
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axin 2
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Probe:
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Mm-Axin2 (400331)
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Probe preparation:
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Antisense
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Visualized with:
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Chromogenic
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Assay notes:
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RNAscope-Red assay.
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Results
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Specimen
1E inset:
embryonic day 14.5
(more  )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1E inset:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tensor veli palatini |
Present |
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1E inset
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In the tensor veli palatini region, expression was predominantly present in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive muscle fibers.
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Specimen
1F inset:
embryonic day 14.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1F inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatoglossus |
Present |
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1F inset
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In the palatoglossus region, expression was abundant in cranial neural crest-derived mesenchymal cells, with the highest expression in the region close to the epithelium. A few labeled myogenic cells were also found.
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Specimen
1G inset:
embryonic day 14.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1G inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: levator veli palatini |
Present |
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1G inset
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In the levator veli palatini region, expression was predominantly present in cranial neural crest-derived mesenchymal cells.
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Specimen
1H inset:
embryonic day 14.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1H inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: soft palate |
Present |
Regionally restricted |
1H inset
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In the putative palatopharyngeus region, expression was detected in cranial neural crest-derived mesenchymal cells.
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Specimen
1I inset:
embryonic day 16.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1I inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: tensor veli palatini |
Present |
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1I inset
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In the tensor veli palatini region, expression was predominantly present in cranial neural crest-derived mesenchymal cells.
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Specimen
1J inset:
embryonic day 16.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1J inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: palatoglossus |
Present |
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1J inset
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In the palatoglossus region, expression was predominantly present in cranial neural crest-derived mesenchymal cells.
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Specimen
1K inset:
embryonic day 16.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1K inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: levator veli palatini |
Present |
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1K inset
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In the levator veli palatini region, expression was predominantly present in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive cells.
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Specimen
1L inset:
embryonic day 16.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1L inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: soft palate |
Present |
Regionally restricted |
1L inset
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In the palatopharyngeus region, expression was predominantly present in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive cells.
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Specimen
1M inset:
embryonic day 18.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1M inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tensor veli palatini |
Present |
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1M inset
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In the tensor veli palatini region, expression persisted in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive myogenic fibers.
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Specimen
1N inset:
embryonic day 18.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1N inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: palatoglossus |
Present |
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1N inset
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In the palatoglossus region, expression persisted in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive myogenic fibers.
|
Specimen
1O inset:
embryonic day 18.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1O inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: levator veli palatini |
Present |
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1O inset
|
In the levator veli palatini region, expression persisted in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive myogenic fibers.
|
Specimen
1P inset:
embryonic day 18.5
(more )
Note: Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
Specimen
1P inset:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: pink - Axin2; brown - Myosin Heavy Chain protein.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: soft palate |
Present |
Regionally restricted |
1P inset
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In the palatopharyngeus region, expression persisted in cranial neural crest-derived mesenchymal cells and in a few myosin heavy chain (MHC)-positive myogenic fibers.
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Analysis Tools