Antibody:	anti-Calbindin D28k (CB-38)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present	Regionally restricted	4C	Immunopositive interneurons were seen in the preplate and intermediate zone migratory streams.
TS21: cortical preplate	Present	Regionally restricted	4C	Immunopositive interneurons were seen in the preplate and intermediate zone migratory streams.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present	Regionally restricted	4D	Immunopositive interneurons had migrated a significantly shorter distance in the preplate and intermediate zone migratory streams in the mutant compared with control littermates.
TS21: cortical preplate	Present	Regionally restricted	4D	Immunopositive interneurons had migrated a significantly shorter distance in the preplate and intermediate zone migratory streams in the mutant compared with control littermates.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		5D inset	Expression was detected in the three migratory streams, which includes Layer 1 migratory stream.
TS24: cerebral cortex subventricular zone	Present		5D inset	Expression was detected in the three migratory streams.
TS24: cortical subplate	Present		5D inset	Expression was detected in the three migratory streams.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		5E inset	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
TS24: cerebral cortex subventricular zone	Present		5E inset	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
TS24: cortical subplate	Present		5E inset	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Uniform	8G	In the control cortex, early-born (Calb1/BrdU+E12.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical plate	Present	Uniform	8G	In the control cortex, early-born (Calb1/BrdU+E12.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical subplate	Present	Uniform	8G	In the control cortex, early-born (Calb1/BrdU+E12.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Weak		8K	Few cells made it to the cortical plate in the mutant.
TS26: cerebral cortex marginal zone	Present		8K	The majority of immunostained cells, particularly those born early, were found in layer I.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Uniform	8H	In the control cortex, early-born (Calb1/BrdU+E13.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical plate	Present	Uniform	8H	In the control cortex, early-born (Calb1/BrdU+E13.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical subplate	Present	Uniform	8H	In the control cortex, early-born (Calb1/BrdU+E13.5) cells were distributed evenly in the Layer I, cortical plate and subplate layers.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Weak		8L	Few cells made it to the cortical plate in the mutant.
TS26: cerebral cortex marginal zone	Present		8L	The majority of immunostained cells, particularly those born early, were found in layer I.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Uniform	8I	In the control cortex, late-born (Calb1/BrdU+E15.5) cells were still distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical plate	Present	Uniform	8I	In the control cortex, late-born (Calb1/BrdU+E15.5) cells were still distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cortical subplate	Present	Uniform	8I	In the control cortex, late-born (Calb1/BrdU+E15.5) cells were still distributed evenly in the Layer I, cortical plate and subplate layers.
TS26: cerebral cortex subventricular zone	Present		8I	Most of the late-born immunopositive cells still resided in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Weak		8M	Few cells made it to the cortical plate in the mutant.
TS26: cerebral cortex marginal zone	Present		8M	An increase in the presence of immunopositive late-born neurons was also observed in Layer I in the mutant.
TS26: cerebral cortex intermediate zone	Present		8M	A significant accumulation of late-born, but not early-born, cells was found in the intermediate zone.