Antibody:	anti-Calbindin D28k (CB-38)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2A	Expression was detected in a subpopulation of cortical interneurons and some pyramidal cells. The majority of labeled cells populated the lower cortical layers, particularly layer V.
TS28: cortical layer V	Present		2A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2B	Labeled cells resided mostly superficially in mutant cortex.
TS28: cortical layer I	Present		2B	A significant increase in proportion of immunopositive cells accumulated in layer I of mutant mice compared with controls.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	4A	Immunopositive interneurons migrate into the cortex from the ganglionic eminence in two streams (pre-plate and intermediate zone).
TS21: ganglionic eminence	Present		4A

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	4B	Fewer immunopositive interneurons in mutant mice had entered the cortex from the medial ganglionic eminence than seen in control littermates. The distance traveled by immunopositive cells in the preplate and intermediate zone migratory streams was a significantly shorter distance in the mutant compared with controls.
TS21: ganglionic eminence	Present		4B

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present			Markedly fewer immunopositive cells had migrated into the developing mutant hippocampus compared to controls at E14.5.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present		4I

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present		4J	Expression was detected in cells with an increased branching morphology in the mutant.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		5D	Expression was detected in the three migratory streams, which includes Layer 1 migratory stream.
TS24: cerebral cortex subventricular zone	Present		5D	Expression was detected in the three migratory streams.
TS24: cortical subplate	Present		5D	Expression was detected in the three migratory streams.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex marginal layer	Present		5E	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
TS24: cerebral cortex subventricular zone	Present		5E	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.
TS24: cortical subplate	Present		5E	At E16.5, there was no difference in the distribution of immunopositive cells between control and mutant mice, and the total number of labeled neurons was similar in the two genotypes.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present		6A
TS26: cortical plate	Present		6A
TS26: cortical subplate	Present		6A
TS26: cerebral cortex intermediate zone	Weak		6A

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present		6B	Immunopositive cells accumulated extensively in Layer I of the mutant cortex.
TS26: cortical plate	Present		6B
TS26: cortical subplate	Present		6B
TS26: cerebral cortex intermediate zone	Present		6B	A slight but significant increase in the number of immunopositive cells was observed in the intermediate zone of the mutant.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Uniform	6D	Expression was evenly distributed in the layers.
TS26: cortical plate	Present	Uniform	6D	Expression was evenly distributed in the layers.
TS26: cortical subplate	Present	Uniform	6D	Expression was evenly distributed in the layers.
TS26: cerebral cortex intermediate zone	Weak		6D

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present		6G	Immunopositive cells accumulated extensively in Layer I of the mutant cortex.
TS26: cortical plate	Present		6G
TS26: cortical subplate	Present		6G
TS26: cerebral cortex intermediate zone	Present		6G	A slight but significant increase in the number of immunopositive cells was observed in the intermediate zone of the mutant.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Non-Uniform	6J	The majority of immunopositive cells were in layer V in controls.
TS27: cortical layer V	Present		6J

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	6K	The majority of staining was in the upper cortex in the mutant.
TS27: cortical layer I	Present		6K

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Non-Uniform	6L	The majority of immunopositive cells were in layer V in controls.
TS27: cortical layer V	Present		6L

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	6M	The majority of staining was in the upper cortex in the mutant.
TS27: cortical layer I	Present		6M

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present		7B	In the Cdk5-/- cortex, layer I showed an abnormal accumulation of immunopositive interneurons.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	7E	The reeler mutant superplate contained an abundance of immunopositive cells.