Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135151
Sigulinsky CL, et al., Vsx2/Chx10 ensures the correct timing and magnitude of Hedgehog signaling in the mouse retina. Dev Biol. 2008 May 15;317(2):560-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6383015
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe157
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The genetic background of the wild type specimens was either +/+ or heterozygous orJ. The authors report these mice to be phenotypically indistinguishable and considered to be equivalent.
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Results
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Specimen
E12.5 wild type not shown:
embryonic day 12.5
(more  )
Specimen
E12.5 wild type not shown:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Ambiguous |
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Authors report expression difficult to detect.
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Specimen
E12.5 orJ not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Ambiguous |
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Authors report expression difficult to detect.
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Specimen
2A:
embryonic day 15.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Widespread |
2A
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Expression in nascent retinal ganglion cell (RGC) layer extending to peripheral retina.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Vsx2or-J/Vsx2or-J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
2B
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Expression restricted to central retina.
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Analysis Tools
