Gata4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:127540 Margagliotti S, et al., The Onecut transcription factors HNF-6/OC-1 and OC-2 regulate early liver expansion by controlling hepatoblast migration. Dev Biol. 2007 Nov 15;311(2):579-89
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6379993
Gene symbol:	Gata4
Gene name:	GATA binding protein 4

Antibody:	anti-Gata-4
Detection system:	Alexa Fluor 594

Results

2I ©

Specimen 2I: embryonic day 9.0 (more

)
Note: 19 somites. Control embryos were either wild-type or Hnf6+/+;Oc2+/lacZ.

Structure	Level	Pattern	Image	Note
TS14: septum transversum	Present		2I
TS14: hepatic primordium	Absent		2I

2J ©

Specimen 2J: embryonic day 9.0; Onecut1^tm1Flem/Onecut1^tm1Flem, Onecut2^tm1Flem/Onecut2^tm1Flem (more

)
Note: 18 somites. Hnf6/Oc2-deficient embryos were either Hnf6-/-;Oc2+/lacZ or Hnf6-/-; Oc2lacZ/lacZ.

Structure	Level	Pattern	Image	Note
TS14: septum transversum	Present		2J	Hepatoblast colonize liver lobes, but form clusters.
TS14: hepatic primordium	Absent		2J	Hepatoblast colonize liver lobes, but form clusters.

2M ©

Specimen 2M: embryonic day 9.5 (more

)
Note: 24 somites. Control embryos were either wild-type or Hnf6+/+;Oc2+/lacZ. Double labeled: red - Gata4; green - TUNEL staining.

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present		2M

2N ©

Specimen 2N: embryonic day 9.5; Onecut1^tm1Flem/Onecut1^tm1Flem, Onecut2^tm1Flem/Onecut2^tm1Flem (more

)
Note: 24 somites. Hnf6/Oc2-deficient embryos were either Hnf6-/-;Oc2+/lacZ or Hnf6-/-; Oc2lacZ/lacZ. Double labeled: red - Gata4; green - TUNEL staining.

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present		2N

2Q ©

Specimen 2Q: embryonic day 9.5 (more

)
Note: 29 somites. Control embryos were either wild-type or Hnf6+/+;Oc2+/lacZ. Double labeled: red - Gata4; green - phosphohistone H3.

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present		2Q

2R ©

Specimen 2R: embryonic day 9.5; Onecut1^tm1Flem/Onecut1^tm1Flem, Onecut2^tm1Flem/Onecut2^tm1Flem (more

)
Note: 29 somites. Hnf6/Oc2-deficient embryos were either Hnf6-/-;Oc2+/lacZ or Hnf6-/-; Oc2lacZ/lacZ. Double labeled: red - Gata4; green - phosphohistone H3.

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present		2R

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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