Tgfbr1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74181 Chow JF, et al., Quantification of transforming growth factor beta1 (TGFbeta1) mRNA expression in mouse preimplantation embryos and determination of TGFbeta receptor (type I and type II) expression in mouse embryos and reproductive tract. Mol Hum Reprod. 2001 Nov;7(11):1047-56
Assay type:	Northern blot
MGI Accession ID:	MGI:6379220
Gene symbol:	Tgfbr1
Gene name:	transforming growth factor, beta receptor I

Probe:	ALK-5 probe2
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 4A Tbeta RI

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	5.4 kb	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Oviduct D1	P adult	TS28: oviduct	Present (a)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 1 of pregnancy.
Oviduct D2	P adult	TS28: oviduct	Present (a)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 2 of pregnancy.
Oviduct D3	P adult	TS28: oviduct	Present (a)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 3 of pregnancy.
Oviduct D4	P adult	TS28: oviduct	Present (a)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 4 of pregnancy.
Uterus D1	P adult	TS28: uterus	Weak (b)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 1 of pregnancy.
Uterus D2	P adult	TS28: uterus	Present (c)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 2 of pregnancy.
Uterus D3	P adult	TS28: uterus	Strong (d)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 3 of pregnancy.
Uterus D4	P adult	TS28: uterus	Weak (e)	2 µg; total RNA	involves: A2G * C57BL/6J		Female	Day 4 of pregnancy.

Notes:
(a) Expression levels in oviduct remain virtually unaltered from days 1-4 of pregnancy.
(b) Low expression on day 1 of prenancy.
(c) Expression increased gradually on day 2.
(d) Nearly 3.5-fold increase in expression on day 3.
(e) Experssion level dropped on day 4.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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