Antibody:	anti-Zic2
Assay notes:	Authors state the distribution of protein expression corresponded to regions in which Zic2 and Zic3 mRNA were detected. Both genes have been annotated.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	1S	Expression located in nuclei of ectodermal and mesodermal cells.
TS10: embryo mesoderm	Present	Regionally restricted	1S	Expression located in nuclei of ectodermal and mesodermal cells.
TS10: extraembryonic component	Present	Regionally restricted	1S	Expression located in nuclei of ectodermal and mesodermal cells.
TS10: primitive streak	Present		1S

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1T	Expression located in nuclei of ectodermal and mesodermal cells.
TS11: embryo mesoderm	Present	Regionally restricted	1T	Expression located in nuclei of ectodermal and mesodermal cells.
TS11: extraembryonic component	Present	Regionally restricted	1T	Expression located in nuclei of ectodermal and mesodermal cells.
TS11: head mesenchyme	Present		1T
TS11: neural fold	Present		1T
TS11: primitive streak	Present		1T

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present		1U
TS11: primitive streak	Present		1U

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present		1V
TS13: future spinal cord neural fold	Present		1V

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Weak	Regionally restricted	2O	Weak expression in anterior S-1 (prospective somite).
TS17: tail somite	Strong	Regionally restricted	2O	Strong expression in S0 (forming somite) and S1 (most newly formed somite).

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Weak	Regionally restricted	2P	Weak expression in anterior S-1 (prospective somite).
TS17: tail somite	Strong	Regionally restricted	2P	Strong expression in S0 (forming somite) and S1 (most newly formed somite).

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Weak	Regionally restricted	S1C	Weak expression in anterior S-1 (prospective somite).
TS17: tail somite	Strong	Regionally restricted	S1C	Strong expression in S0 (forming somite) and S1 (most newly formed somite). Occasional expression in S2.