Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:222465
Borello U, et al., Sp8 and COUP-TF1 reciprocally regulate patterning and Fgf signaling in cortical progenitors. Cereb Cortex. 2014 Jun;24(6):1409-21
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6377228
|
Gene symbol:
|
Sp8
|
Gene name:
|
trans-acting transcription factor 8
|
Antibody:
|
Anti-Sp8
|
Detection system:
|
Secondary antibody coupled to Cy2
|
|
Results
|
Specimen
1A:
embryonic day 9.5
(more )
Note: Double labeled: green - Sp8; red - Nr2f1 (assay MGI:6377229).
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Sp8; red - Nr2f1 (assay MGI:6377229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: telencephalon |
Present |
Graded |
1A
|
Expression was detected in a gradient of high rostral to low caudal. The staining was complementary to that of Nr2f1.
|
Specimen
1B:
embryonic day 12.5
(more )
Note: Double labeled: green - Sp8; red - Nr2f1 (assay MGI:6377229).
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Sp8; red - Nr2f1 (assay MGI:6377229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Graded |
1B
|
Expression was detected in a gradient of high rostral to low caudal. Along the dorsoventral axis of the pallium, expression was detected in a high-dorsal to low-ventral gradient. The staining was complementary to that of Nr2f1 in both the rostrocaudal and dorsoventral axis.
|
|