Probe:	Zic3 probe5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present		1J
TS10: embryo mesoderm	Present		1J

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		1K
TS11: embryo mesoderm	Present		1K

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		1L
TS11: embryo mesoderm	Present		1L

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1M	Expression in dorsal neural tube.
TS12: somite	Present	Regionally restricted	1M	Expresison in somites, with enhancement in caudal segments.
TS12: unsegmented mesenchyme	Present		1M

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1N	Expression in dorsal neural tube.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present	Regionally restricted	1O	Expression in dorsal neural tube in hindbrain regions (rhombomeres 1, 2 and 4)
TS13: somite	Present	Regionally restricted	1O	Expression in somites, with enhancement in caudal segments.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Present		1P
TS13: rhombomere 2	Present		1P
TS13: rhombomere 4	Present		1P
TS13: somite	Present	Regionally restricted	1P	Expresison in somites, with enhancement in caudal segments.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		1Q
TS14: rhombomere 2	Present		1Q
TS14: rhombomere 4	Present		1Q
TS14: somite	Present	Regionally restricted	1Q	Expresison in somites, with enhancement in caudal segments.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 1	Present		1R
TS14: rhombomere 2	Present		1R
TS14: rhombomere 4	Present		1R
TS14: somite	Present	Regionally restricted	1R	Expresison in somites, with enhancement in caudal segments.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2D	In the tail, broad expression throughout presomitic mesoderm and somites. Single stripe in anterior presomitic mesoderm with gradient to the posterior end.
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2D	In the tail, broad expression throughout presomitic mesoderm and somites. Single stripe in anterior presomitic mesoderm with gradient to the posterior end.
TS17: somite	Present	Regionally restricted	2D	In segmented somites, expression distributed evenly rostro-caudally.
TS17: tail neural tube	Absent		2D

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2L	In the tail, broad expression throughout presomitic mesoderm . Single stripe in anterior presomitic mesoderm coinciding with S-1 (prospective) with gradient to the posterior end.
TS17: tail somite	Present	Regionally restricted	2L	In segmented somites, expression distributed evenly rostro-caudally.
TS17: tail neural tube	Absent		2L

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2M	In the tail, broad expression throughout presomitic mesoderm and somites. Single stripe in anterior presomitic mesoderm coinciding with S-1 (prospective somite) with gradient to the posterior end.
TS17: tail somite	Present	Regionally restricted	2M	In segmented somites, expression distributed evenly rostro-caudally.
TS17: tail neural tube	Absent		2M

Structure	Level	Pattern	Image	Note
TS17: tail neural tube	Absent		2N

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present		S1B
TS17: tail unsegmented mesenchyme	Present		S1B