Probe:	Zic2 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Widespread	1A	Expressed broadly in both extraembryonic and embryonic components of egg cylinder.
TS10: extraembryonic component	Present	Widespread	1A	Expressed broadly in both extraembryonic and embryonic components of egg cylinder.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	1B	Increased expression, first in anterior ectoderm, then in mesenchyme of headfold.
TS11: head mesenchyme	Present	Regionally restricted	1B	Increased expression, first in anterior ectoderm, then in mesenchyme of headfold.

Structure	Level	Pattern	Image	Note
TS11: head mesenchyme	Present	Regionally restricted	1C	Increased expression in mesoderm of headfold.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	1D	Expression in dorsal neural folds.
TS12: somite	Present	Regionally restricted	1D	Expression in somites, with enhancements in a few caudal somites
TS12: unsegmented mesenchyme	Present	Regionally restricted	1D	Expression in anterior presomitic mesoderm, .

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	1E	Expression in dorsal neural folds.
TS12: somite	Present	Regionally restricted	1E	Expression in somites, with enhancements in a few caudal somites
TS12: unsegmented mesenchyme	Present	Regionally restricted	1E	Expression in anterior presomitic mesoderm, .

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1F	Expression in dorsal neural folds.
TS13: somite	Present	Regionally restricted	1F	Expression in somites, with enhancements in a few caudal somites.
TS13: unsegmented mesenchyme	Present	Regionally restricted	1F	Expression in anterior presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Present	Regionally restricted	1G	Expression in dorsal neural folds.
TS13: somite	Present	Regionally restricted	1G	Expression in somites, with enhancements in a few caudal somites
TS13: unsegmented mesenchyme	Present	Regionally restricted	1G	Expression in anterior presomitic mesoderm, .

Structure	Level	Pattern	Image	Note
TS14: neural fold	Present	Regionally restricted	1H	Expression in dorsal neural folds.
TS14: somite	Present	Regionally restricted	1H	Expression in somites, with enhancements in a few caudal somites
TS14: unsegmented mesenchyme	Present	Regionally restricted	1H	Expression in anterior presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS14: neural fold	Present	Regionally restricted	1I	Expression in dorsal neural folds.
TS14: somite	Present	Regionally restricted	1I	Expression in somites, with enhancements in a few caudal somites
TS14: unsegmented mesenchyme	Present	Regionally restricted	1I	Expression in anterior presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2A	In 64% of embryos, expression in presomitic mesoderm and somite detected as 2 distinct stripes.
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2A	In 64% of embryos, expression in presomitic mesoderm and somite detected as 2 distinct stripes.
TS17: tail neural tube	Present	Regionally restricted	2A	Expression in caudal end of neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2B	In 64% of embryos, expression in presomitic mesoderm and somite detected as 2 distinct stripes. In 2 of 19 embryos examined, expression in S1 (most newly formed somite) was weaker than in S0 (forming somite).
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2B
TS17: tail neural tube	Present	Regionally restricted	2B	Expression in caudal end of neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2C	In 5 of 19 embryo examined, expression as a single broad stripe in which anterior half of stripe corresponded to S0 (forming somite) and posterior half of stripe to S-1 (prospective somite).
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2C	In 5 of 19 embryo examined, expression as a single broad stripe in which anterior half of stripe corresponded to S0 (forming somite) and posterior half of stripe to S-1 (prospective somite).
TS17: tail neural tube	Present	Regionally restricted	2C	Expression in caudal end of neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2I	Two stripes of expression corresponding to S0 (forming somite) and S1 (most newly formed somite). Weaker expression in segmented somites with slight enhancement in anterior compartments.
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2I	Weak expression in anterior part of S-1 (prospective somite).
TS17: tail neural tube	Present	Regionally restricted	2I	Expression in caudal end of neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	2J	Single broad stripe in which anterior half of stripe corresponded to S0 (forming somite). Weaker expression in segmented somites with slight enhancement in anterior compartments.
TS17: tail unsegmented mesenchyme	Present	Regionally restricted	2J	Single broad stripe in which posterior half of stripe corresponded to S-1 (prospective somite).
TS17: tail neural tube	Present	Regionally restricted	2J	Expression in caudal end of neural tube.

Structure	Level	Pattern	Image	Note
TS17: tail neural tube	Present		2K

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present	Regionally restricted	S1A	Expression pattern mostly consistent with that of Zic protein localization. Expression in S0 (forming somite) and S1 (most newly formed somite).