Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119875
Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6368135
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Probe:
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Ngn2 probe18
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
5B:
embryonic day 10.5
(more  )
Specimen
5B:
(close  )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5B
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Exprssion in ventral interneurons of hindbrain, and in a subset of dorsal interneurons.
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5D
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No expression in ventral domain at all levels of hindbrain and spinal cord.
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Specimen
5I:
embryonic day 10.5
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
5I:
(close )
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Genetic Background:
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involves: CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5I
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Dorsal expression domain is substantially broader in rhombomeres 6-7 than in rhombomeres 3-5.
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Specimen
5J:
embryonic day 10.5; Shhtm1Chg/ Shhtm1Chg
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
5J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5J
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Dorsal expression domain shifted ventrally along hindbrain midline.
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Specimen
5K:
embryonic day 10.5; Gli2tm1Alj/ Gli2tm1Alj
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
5K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5K
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Ventral expression domain showed a gradual ventral shirft along the anterior-posterior axis.
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| TS17: rhombomere 1 |
Absent |
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5K
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| TS17: rhombomere 2 |
Present |
Regionally restricted |
5K
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Expression spanning midline of rhombomere 3.
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| TS17: rhombomere 4 |
Present |
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5K
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Expression in rhombomere 2 less affected than in surrounding rhombomeres.
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Specimen
5L:
embryonic day 10.5; Gli3Xt-J/ Gli3Xt-J
(more )
Note: After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
Specimen
5L:
(close )
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Genetic Background:
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involves: C3H/HeJ * CD-1
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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After hybridization as whole mounts, hindbrains were dissected out and flattened opened under a coverslip.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
5L
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Authors report ventral expression domain was affected in the anterior hindbrain.
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| TS17: rhombomere 1 |
Strong |
Regionally restricted |
5L
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Expression domain expanded dorsally at rhombomere 1.
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| TS17: rhombomere 2 |
Strong |
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5L
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Increased expression at rhombomeres 1, 2 and 3.
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| TS17: rhombomere 3 |
Strong |
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5L
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Increased expression at rhombomeres 1, 2 and 3.
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Analysis Tools