Isl1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:119875 Lebel M, et al., Gli2 and Gli3 play distinct roles in the dorsoventral patterning of the mouse hindbrain. Dev Biol. 2007 Feb 1;302(1):345-55
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6368120
Gene symbol:	Isl1
Gene name:	ISL1 transcription factor, LIM/homeodomain

Antibody:	anti-Isl1/2
Detection system:	horseradish peroxidase

Results

2U ©

Specimen 2U: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	2U	Expression in motoneurons in ventral spinal cord.

2V ©

Specimen 2V: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent		2V	Authors state montoneurons are absent.

2W ©

Specimen 2W: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak	Regionally restricted	2W	Expression in motoneurons in ventral spinal cord shifted ventrally and reduced in number.

2X ©

Specimen 2X: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent		2X	Authors state montoneurons are absent.

2Y ©

Specimen 2Y: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J, Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Weak	Regionally restricted	2Y	Expression in motoneurons in ventral spinal cord shifted ventrally and reduced in number.

2Z ©

Specimen 2Z: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Present	Regionally restricted	2Z	Authors state hindbrain expression was similar to that observed in the spinal cord.

2AA ©

Specimen 2AA: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Absent		2AA	Authors state hindbrain expression was similar to that observed in the spinal cord.

2BB ©

Specimen 2BB: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Ambiguous		2BB	No or very few expressing cells detected.

2CC ©

Specimen 2CC: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Absent		2CC	Authors state hindbrain expression was similar to that observed in the spinal cord.

2DD ©

Specimen 2DD: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J, Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Ambiguous		2DD	No or very few expressing cells detected.

S3F r2 ©

Specimen S3F r2: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Present		S3F r2	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3F r4 ©

Specimen S3F r4: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present		S3F r4	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3F r5 ©

Specimen S3F r5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Present		S3F r5	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3F r7 ©

Specimen S3F r7: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Present		S3F r7	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3G r2 ©

Specimen S3G r2: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Absent		S3G r2	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3G r4 ©

Specimen S3G r4: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Absent		S3G r4	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3G r5 ©

Specimen S3G r5: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Absent		S3G r5	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3G r7 ©

Specimen S3G r7: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Absent		S3G r7	Authors state hindbrain expression was similar to that observed in the spinal cord.

S3H r2 ©

Specimen S3H r2: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Ambiguous		S3H r2	No or very few expressing cells detected.

S3H r4 ©

Specimen S3H r4: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Ambiguous		S3H r4	No or very few expressing cells detected.

S3H r5 ©

Specimen S3H r5: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Ambiguous		S3H r5	No or very few expressing cells detected.

S3H r7 ©

Specimen S3H r7: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Ambiguous		S3H r7

S3I r2 ©

Specimen S3I r2: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Present		S3I r2

S3I r4 ©

Specimen S3I r4: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Present		S3I r4

S3I r5 ©

Specimen S3I r5: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Present		S3I r5

S3I r7 ©

Specimen S3I r7: embryonic day 10.5; Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Present		S3I r7

S3J r2 ©

Specimen S3J r2: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Ambiguous		S3J r2	No or very few expressing cells detected.

S3J r4 ©

Specimen S3J r4: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Ambiguous		S3J r4	No or very few expressing cells detected.

S3J r5 ©

Specimen S3J r5: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 5	Ambiguous		S3J r5	No or very few expressing cells detected.

S3J r7 ©

Specimen S3J r7: embryonic day 10.5; Gli2^tm1Alj/Gli2^tm1Alj, Gli3^Xt-J/Gli3^Xt-J (more

)

Structure	Level	Pattern	Image	Note
TS17: rhombomere 7	Ambiguous		S3J r7	No or very few expressing cells detected.

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