Th RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:118802 Blak AA, et al., Fgfr2 and Fgfr3 are not required for patterning and maintenance of the midbrain and anterior hindbrain. Dev Biol. 2007 Mar 1;303(1):231-43
Assay type:	RNA in situ
MGI Accession ID:	MGI:6363697
Gene symbol:	Th
Gene name:	tyrosine hydroxylase

Probe:	Th probe12
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Results

2Ba ©

Specimen 2Ba: embryonic day 12.5; Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present	Regionally restricted	2Ba	Expression in floor plate and basal plate of ventral midbrain, marking future dopaminergic neurons of midbrain.

2Ba' ©

Specimen 2Ba': embryonic day 12.5; En1^tm2(cre)Wrst/En1⁺, Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present	Regionally restricted	2Ba'	Expression unchanged compared to control.

3Aa ©

Specimen 3Aa: embryonic day 12.5; Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3Aa	Expression in ventral midbrain, marking presumptive dopaminiergic neurons of midbrain.

3Aa' ©

Specimen 3Aa': embryonic day 12.5; En1^tm2(cre)Wrst/En1⁺, Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3Aa'	Expression unchanged compared to control.

3Ab ©

Specimen 3Ab: embryonic day 12.5; Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3Ab	Expression in presumptive locus coeruleus (ventral rhombomere 1).

3Ab' ©

Specimen 3Ab': embryonic day 12.5; En1^tm2(cre)Wrst/En1⁺, Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3Ab'	Expression unchanged compared to control.

3Ba ©

Specimen 3Ba: postnatal adult; Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Regionally restricted	3Ba	Expression in dopaminergic neurons of substantia nigra and ventral tegmental area.
TS28: ventral tegmental area	Present	Regionally restricted	3Ba	Expression in dopaminergic neurons of substantia nigra and ventral tegmental area.

3Ba' ©

Specimen 3Ba': postnatal adult; En1^tm2(cre)Wrst/En1⁺, Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Regionally restricted	3Ba'	Expression unchanged compared to control.
TS28: ventral tegmental area	Present	Regionally restricted	3Ba'	Expression unchanged compared to control.

3Bb ©

Specimen 3Bb: postnatal adult; Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present	Regionally restricted	3Bb	Expression in noradrenergic neuros of the locus coeruleus.

3Bb' ©

Specimen 3Bb': postnatal adult; En1^tm2(cre)Wrst/En1⁺, Fgfr2^tm1Wrst/Fgfr2^tm1Wrst (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present	Regionally restricted	3Bb'	Expression unchanged compared to control.

4Ba ©

Specimen 4Ba: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Regionally restricted	4Ba	Expression in dopaminergic neurons of substantia nigra and ventral tegmental area.
TS28: ventral tegmental area	Present	Regionally restricted	4Ba	Expression in dopaminergic neurons of substantia nigra and ventral tegmental area.

4Ba' ©

Specimen 4Ba': postnatal adult; Fgfr3^tm1Dor/Fgfr3^tm1Dor (more

)

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Regionally restricted	4Ba'	Expression unchanged compared to control.
TS28: ventral tegmental area	Present	Regionally restricted	4Ba'	Expression unchanged compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24