T RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:108723 Andersson O, et al., Synergistic interaction between Gdf1 and Nodal during anterior axis development. Dev Biol. 2006 May 15;293(2):370-81
Assay type:	RNA in situ
MGI Accession ID:	MGI:6363328
Gene symbol:	T
Gene name:	brachyury, T-box transcription factor T

Probe:	T probe35
Probe preparation:	labelled with digoxigenin

Results

2C WT ©

Specimen 2C WT: embryonic day 7.0 (more

)
Note: Early Allantoic Bud

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	2C WT	Expression in mesodermal cells along primitive streak.
TS10: embryo mesoderm	Present	Regionally restricted	2C WT	Expression in axial mesendoderm.

2C G-/- ©

Specimen 2C G-/-: embryonic day 7.0; Gdf1^tm1Sjl/Gdf1^tm1Sjl (more

)
Note: Early Allantoic Bud

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2C G-/-	Expression in cells in the streak.
TS10: embryo mesoderm	Present	Regionally restricted	2C G-/-	Single mutant embryos did not show defects in axial mesendoderm.

2C N+/- ©

Specimen 2C N+/-: embryonic day 7.0; Nodal^tm1Rob/Nodal^tm1Rob (more

)
Note: Early Allantoic Bud

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2C N+/-	Expression in cells in the streak.
TS10: embryo mesoderm	Present	Regionally restricted	2C N+/-	Single mutant embryos did not show defects in axial mesendoderm.

2C G-/- N+/- (left) ©

Specimen 2C G-/- N+/- (left): embryonic day 7.0; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)
Note: Early Allantoic Bud

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2C G-/- N+/- (left)	Expression in cells in the streak.
TS10: embryo mesoderm	Absent		2C G-/- N+/- (left)	Expression greatly reduced in half of mutant embryos examined.

2C G-/- N+/- (right) ©

Specimen 2C G-/- N+/- (right): embryonic day 7.0; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)
Note: Early Allantoic Bud

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		2C G-/- N+/- (right)

2E WT ©

Specimen 2E WT: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		2E WT
TS13: primitive streak	Present		2E WT

2E G-/- N+/- (left) ©

Specimen 2E G-/- N+/- (left): embryonic day 8.5; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	2E G-/- N+/- (left)	Expression in regressing primitive streak.
TS13: notochord	Ambiguous		2E G-/- N+/- (left)	Expression often partially or completely missing in notochord.

2E G-/- N+/- (right) ©

Specimen 2E G-/- N+/- (right): embryonic day 8.5; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	2E G-/- N+/- (right)	Expression in regressing primitive streak.
TS13: notochord	Ambiguous		2E G-/- N+/- (right)	Expression often partially or completely missing in notochord.

2F ©

Specimen 2F: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		2F

2G ©

Specimen 2G: embryonic day 8.5; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		2G	Expression in regressing primitive streak.

2H ©

Specimen 2H: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		2H

2I ©

Specimen 2I: embryonic day 8.5; Gdf1^tm1Sjl/Gdf1^tm1Sjl, Nodal^tm1Rob/Nodal⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: chordamesoderm	Absent		2I	Authors state notochord was absent in most severely affected embryos.

7E WT ©

Specimen 7E WT: embryonic day 7.5 (more

)
Note: Late Allantoic Bud

Structure	Level	Pattern	Image	Note
TS11: axial mesoderm	Present		7E WT
TS11: primitive streak	Present		7E WT

7E G-/- ©

Specimen 7E G-/-: embryonic day 7.5; Gdf1^tm1Sjl/Gdf1^tm1Sjl (more

)
Note: Late Allantoic Bud

Structure	Level	Pattern	Image	Note
TS11: axial mesoderm	Present		7E G-/-	Single mutants did not show decreased expression in axial mesendoderm.

7E 4+/- ©

Specimen 7E 4+/-: embryonic day 7.5; Acvr1b^tm1Enl/Acvr1b⁺ (more

)
Note: Late Allantoic Bud

Structure	Level	Pattern	Image	Note
TS11: axial mesoderm	Present		7E 4+/-	Single mutants did not show decreased expression in axial mesendoderm.

7E 4+/- G-/- ©

Specimen 7E 4+/- G-/-: embryonic day 7.5; Acvr1b^tm1Enl/Acvr1b⁺, Gdf1^tm1Sjl/Gdf1^tm1Sjl (more

)
Note: Late Allantoic Bud

Structure	Level	Pattern	Image	Note
TS11: axial mesoderm	Weak	Regionally restricted	7E 4+/- G-/-	Partial loss of expression in axial mesendoderm of a subset of double mutant embryos.

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