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Gene Expression Data
Assay Details
Assay
Reference: J:108393 Chung AC, et al., Loss of orphan nuclear receptor GCNF function disrupts forebrain development and the establishment of the isthmic organizer. Dev Biol. 2006 May 1;293(1):13-24
Assay type: RNA in situ
MGI Accession ID: MGI:6360876
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 cDNA13
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3A: embryonic day 8.5 (more )
Note: 6 somites
Structure Level Pattern Image Note
TS12: future midbrain Present 3A
TS12: embryo ectoderm Present Regionally restricted 3A Expression in anterior neural ridge.
Specimen 3B: embryonic day 8.5; Nr6a1tm1Coo/Nr6a1tm1Coo (more )
Note: 6 somites
Structure Level Pattern Image Note
TS12: future midbrain Weak 3B Expression reduced in midbrain of mutant embryos.
TS12: embryo ectoderm Trace Regionally restricted 3B Expression significantly reduced in anterior neural ridge.
Specimen 3C: embryonic day 9.5 (more )
Note: 25 somites
Structure Level Pattern Image Note
TS15: midbrain-hindbrain junction Present 3C
TS15: embryo ectoderm Present Regionally restricted 3C Expression in anterior neural ridge.
Specimen 3D: embryonic day 9.5; Nr6a1tm1Coo/Nr6a1tm1Coo (more )
Note: 13 somites
Structure Level Pattern Image Note
TS14: future midbrain Trace 3D Expression significantly reduced in midbrain.
TS14: embryo ectoderm Trace Regionally restricted 3D Expression significantly reduced in anterior neural ridge.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
MGI 6.24 		The Jackson Laboratory