Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106405
Mackay DR, et al., The mouse Ovol2 gene is required for cranial neural tube development. Dev Biol. 2006 Mar 1;291(1):38-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6360624
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5I +/-:
embryonic day 8.5; Ovol2tm1Xdai/Ovol2 +
(more  )
Specimen
5I +/-:
(close  )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
5I +/-
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Expression in dorsal aspect of neuroectoderm, restricted to area adjacent to neuroectoderm/surface ectoderm junction.
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Specimen
5I -/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2tm1Xdai
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
5I -/-
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Domain of expression expanded ventrally; dorsal concentration still evident.
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Specimen
5J +/-:
embryonic day 8.5; Ovol2tm1Xdai/Ovol2 +
(more )
Specimen
5J +/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
5J +/-
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Expression in dorsal aspect of neuroectoderm, restricted to area adjacent to neuroectoderm/surface ectoderm junction.
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Specimen
5J -/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2tm1Xdai
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
5J -/-
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Dorsal boundary of expression shifted laterally, consistent with morphologically apparent shift of neuroectoderm/surface ectoderm border.
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Analysis Tools
