Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106405
Mackay DR, et al., The mouse Ovol2 gene is required for cranial neural tube development. Dev Biol. 2006 Mar 1;291(1):38-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6360620
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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pOtd9
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A +/-:
embryonic day 8.5; Ovol2tm1Xdai/Ovol2 +
(more  )
Specimen
5A +/-:
(close  )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
5A +/-
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Expression in neurectoderm and anterior mesendoderm of developing forebrain and midbrain.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
5A +/-
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Expression in neurectoderm and anterior mesendoderm of developing forebrain and midbrain.
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Specimen
5A -/-:
(close )
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Genetic Background:
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either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * CD-1)
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Mutant Allele(s):
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Ovol2tm1Xdai/Ovol2tm1Xdai
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
5A -/-
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Authors report largely normal A-P brain patterning in mutants with forebrain and midbrain domains specified properly.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
5A -/-
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Authors report largely normal A-P brain patterning in mutants with forebrain and midbrain domains specified properly.
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Analysis Tools
