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Gene Expression Data
Assay Details
Assay
Reference: J:27770 Sadler TW, et al., Antisense targeting of engrailed-1 causes abnormal axis formation in mouse embryos. Teratology. 1995 May;51(5):292-9
Assay type: RT-PCR
MGI Accession ID: MGI:6360209
Gene symbol: En1
Gene name: engrailed 1
Probe: En1-pG, En1-pH
Assay notes: The RT-PCR reaction was Southern blotted and probed; see MGI:6360197 for probe details. Probe was labelled with digoxigenin and visualized with alkaline phosphatase.
Results Image: 2C lanes 5&6
 Sample Information Bands Other Sample Information
Lane AgeStructure 387 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
5 E9.0 (a) TS14: head Present Not Specified; total RNA ICR Not Specified 5-14 somites
6 E9.0 (a) TS14: primitive streak
TS14: tail bud 		Present Not Specified; total RNA ICR Not Specified 5-14 somites
- Other lane(s) in blot assay different gene(s)
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) There was also a smaller fragment detected.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory