Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104893
Seo KW, et al., Targeted disruption of the DM domain containing transcription factor Dmrt2 reveals an essential role in somite patterning. Dev Biol. 2006 Feb 1;290(1):200-10
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6359421
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Gene symbol:
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Des
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Gene name:
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desmin
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Results
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Specimen
5A/C:
embryonic day 10.5; Dmrt2tm1Rjo/Dmrt2 +
(more  )
Note: Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
Specimen
5A/C:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5A
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5C
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Expression confined to myotome in a relatively thin medio-lateral band extending dorsally and ventrally.
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Specimen
5B/D:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2tm1Rjo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5B
,
5D
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No expressing cells in dorsal regions of somites. Significantly broader medio-lateral distribution of expression.
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Specimen
5G/I:
embryonic day 10.5; Dmrt2tm1Rjo/Dmrt2 +
(more )
Note: Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
Specimen
5G/I:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5G
,
5I
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Individual expressing myotomal cells extend along entire rostral-caudal extent of myotome
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Specimen
5H/J:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2tm1Rjo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Des; red - Lama1 (assay MGI:6359422); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5J
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5H
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Expression confined to central portion of each myotome.
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Specimen
5M:
embryonic day 10.5; Dmrt2tm1Rjo/Dmrt2 +
(more )
Specimen
5M:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5M
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Individual expressing myotomal cells extend along entire rostral-caudal extent of myotome
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Specimen
5N:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2tm1Rjo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5N
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Expression confined to central portion of each myotome.
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Specimen
5S:
embryonic day 11.5; Dmrt2tm1Rjo/Dmrt2 +
(more )
Specimen
5S:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: somite |
Present |
Regionally restricted |
5S
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Expression in cells in young somite well confined within dorsal-lateral part of omites.
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Specimen
5T:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2tm1Rjo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: somite |
Present |
Regionally restricted |
5T
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Expressing cells show loss of boundary and expand to medial portion of somites.
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Specimen
5W:
embryonic day 13.5; Dmrt2tm1Rjo/Dmrt2 +
(more )
Specimen
5W:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: skeletal muscle |
Present |
Regionally restricted |
5W
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In thoracic region, expression in epaxial, hypaxial and diaphragm muscles.
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Specimen
5X:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Dmrt2tm1Rjo/Dmrt2tm1Rjo
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: skeletal muscle |
Present |
Regionally restricted |
5X
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Expression in epaxial, hypaxial and diaphragm muscles similar to normal.
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Analysis Tools