Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104893
Seo KW, et al., Targeted disruption of the DM domain containing transcription factor Dmrt2 reveals an essential role in somite patterning. Dev Biol. 2006 Feb 1;290(1):200-10
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6359406
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Probe:
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Myog probe11
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3G:
embryonic day 10.5; \ Dmrt2tm1Rjo/\Dmrt2 +
(more  )
Specimen
3G:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2tm1Rjo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: somite |
Weak |
Scattered |
3H
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Expression faded in mature somites. Expressing cells are scattered in mature somites.
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Specimen
3M:
embryonic day 11.5; \ Dmrt2tm1Rjo/\Dmrt2 +
(more )
Specimen
3M:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Regionally restricted |
3M
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Expression in regularly spaced stripes that span the entire dorsal-ventral domain of the myotome.
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| TS19: limb bud |
Present |
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3M
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Specimen
3N:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2tm1Rjo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
Scattered |
3N
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Expressing cells are scattered within somites and less extended toward ventral portion around inter-limb region compared with normal.
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| TS19: limb bud |
Present |
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3N
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Expression in limb region is not changed in mutant.
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Specimen
3O:
embryonic day 11.5; \ Dmrt2tm1Rjo/\Dmrt2 +
(more )
Specimen
3O:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3P:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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\Dmrt2tm1Rjo/\Dmrt2tm1Rjo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: somite |
Present |
Regionally restricted |
3P
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Expressing cells are scattered toward medial portion of somite. Expression in a broader territory compared with wild type.
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Analysis Tools
