Probe:	pEco7a
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present		6A
TS21: brain	Present		6A
TS21: face mesenchyme	Present		6A
TS21: hindlimb	Present		6A
TS21: blood vessel	Present		6A	Expression was detected in large blood vessels.
TS21: metanephros	Weak		6A	Expression in the kidney was not prominent at this stage.

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Strong		7A
TS21: metanephros	Weak	Regionally restricted	7A	A few pretubular aggregates show low levels of expression.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Weak		7B
TS23: renal tubule	Strong	Regionally restricted	7B	Strong signal was evident in differentiating tubular epithelial cells. There was a tendency for tubules that have formed a lumen to express more strongly than those without a lumen.

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Strong	Widespread	7C	Expression in tubular epithelial cells was stronger and more widespread throughout the kidney.
TS24: maturing glomerular tuft	Trace		7C

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Ambiguous			Expression in the adrenal gland is mostly lost at E16.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	8A	Intense expression was detected in the areas surrounding the lateral ventricles.
TS21: cerebral cortex	Present	Regionally restricted	8A	Expression was prominent in the lower half of the cerebral wall. The outer layers are mostly devoid of signal, except for a narrow layer of cells beneath the pia.
TS21: cerebral cortex ventricular layer	Present		8A

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Regionally restricted	8B	Expression was found in the neuroepithelium of the mesencephalic vesicle and the fourth ventricle.
TS21: midbrain	Present	Regionally restricted	8B	Expression was found in the neuroepithelium of the mesencephalic vesicle and the fourth ventricle.
TS21: cerebellum	Present		8B

Structure	Level	Pattern	Image	Note
TS21: ascending aorta	Present		8C
TS21: cardiac muscle tissue	Absent		8C
TS21: heart endothelium	Absent		8C

Structure	Level	Pattern	Image	Note
TS21: blood vessel	Strong	Regionally restricted	8D	Strong labeling appears to coincide with smooth muscle cells in the tunica media. Neither endothelium nor tunica adventitia was labeled.
TS21: intestine smooth muscle	Moderate		8D
TS21: liver	Absent		8D