Gene Expression Data
Assay Details
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Assay
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Reference:
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J:187741
Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6358586
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Gene symbol:
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Dock9
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Gene name:
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dedicator of cytokinesis 9
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Probe:
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Dock9 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
S8K:
embryonic day 11.0
(more  )
Specimen
S8K:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
S8K
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Expression was detected in condensing mesenchyme.
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| TS18: forelimb bud |
Present |
Regionally restricted |
S8K
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Expression was detected in the dorsal and ventral muscle masses.
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Specimen
S8L:
embryonic day 11.0; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S8L:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm2Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
S8L
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Expression was unaffected in condensing mesenchyme in the conditional knockout mutant.
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| TS18: forelimb bud |
Weak |
Regionally restricted |
S8L
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Expression was downregulated in the dorsal and ventral muscle masses in the conditional knockout mutant.
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Specimen
S8M:
embryonic day 11.0
(more )
Specimen
S8M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
S8M
|
Expression was detected in condensing mesenchyme.
|
| TS18: forelimb bud |
Present |
Regionally restricted |
S8M
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Expression was detected in the dorsal and ventral muscle masses.
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Specimen
S8N:
embryonic day 11.0; Smotm2Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S8N:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm2Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
S8N
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Expression was unaffected in condensing mesenchyme in the conditional knockout mutant.
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| TS18: forelimb bud |
Weak |
Regionally restricted |
S8N
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Expression was downregulated in the dorsal and ventral muscle masses in the conditional knockout mutant.
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Analysis Tools