Gene Expression Data
Assay Details
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Assay
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Reference:
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J:187741
Hu JK, et al., Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev. 2012 Sep 15;26(18):2088-102
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6358480
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Results
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Specimen
3O:
(close  )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3O
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Expression was detected in forelimb ventral and dorsal muscle cells.
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Specimen
3P:
embryonic day 10.75; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more  )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3P:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3P
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At E10.75, expression was delayed in the conditional knockout ventral muscle cells. Expression in the dorsal muscle cells was largely unaffected.
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Specimen
3S:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smo+
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
3S
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Expression was detected in forelimb ventral and dorsal muscle cells.
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Specimen
3T:
embryonic day 11.0; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
3T:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
3T
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Expression in forelimb ventral muscle cells had recovered by E11 in the conditional knockout.
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Specimen
S8E:
embryonic day 11.0
(more )
Specimen
S8E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
S8E
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Expression was detected in myocytes.
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Specimen
S8F:
embryonic day 11.0; Smotm1Amc/ Smotm2Amc, Pax3tm1(cre)Joe/Pax3 +
(more )
Note: Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
Specimen
S8F:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Pax3tm1(cre)Joe/Pax3+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. Pax3Cre;SmoCKO specimens can be either Pax3Cre;Smofl/fl or Pax3Cre;Smofl/del.
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Analysis Tools