Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93140 Lu CC, et al., Multiple roles for Nodal in the epiblast of the mouse embryo in the establishment of anterior-posterior patterning. Dev Biol. 2004 Sep 1;273(1):149-59
Assay type:	RNA in situ
MGI Accession ID:	MGI:6342943
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

4C wt ©

Specimen 4C wt: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	4C wt	Expression in anterior mesendoderm.

4C m ©

Specimen 4C m: embryonic day 7.5; Meox2^tm1(cre)Sor/Meox2⁺, Nodal^tm1Rob/Nodal^tm5Rob (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Weak	Regionally restricted	4C m	Expression dramatically reduced compared to wild type.

4E wt ©

Specimen 4E wt: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	4E wt	Midline expression in anterior definitive endoderm.
TS13: notochord	Present		4E wt

4E m ©

Specimen 4E m: embryonic day 8.5; Meox2^tm1(cre)Sor/Meox2⁺, Nodal^tm1Rob/Nodal^tm5Rob (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Weak		4E m	Expression greatly reduced compared to wild type.
TS13: notochord	Weak		4E m	Expression greatly reduced compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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