Dag1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82929 Henion TR, et al., Expression of dystroglycan, fukutin and POMGnT1 during mouse cerebellar development. Brain Res Mol Brain Res. 2003 Apr 10;112(1-2):177-81
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6324921
Gene symbol:	Dag1
Gene name:	dystroglycan 1

Antibody:	alpha-dystroglycan
Detection system:	Secondary antibody coupled to fluorescein

Results

Specimen 2D: postnatal day 7 (more

)
Note: Double labeled: green - Dag1; red - Gfap (assay MGI:6324919).

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	2D alpha-dystroglycan , 2D overlay	Expression is in Bergman glial fibers that pass through the molecular layer and external granule layer.
TS28: cerebellum molecular layer	Present	Regionally restricted	2D alpha-dystroglycan , 2D overlay	Expression is in Bergman glial fibers that pass through the molecular layer and external granule layer.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2D overlay , 2D alpha-dystroglycan	Expression is in Bergman glial fibers.
TS28: cerebellum internal granule cell layer	Present		2D alpha-dystroglycan , 2D overlay

Specimen 2E: postnatal day 7 (more

)
Note: Double labeled: green - Dag1; red - Calb1 (assay MGI:6324923).

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	2E alpha-dystroglycan	Expression is in Bergman glial fibers that pass through the molecular layer and external granule layer.
TS28: cerebellum molecular layer	Present	Regionally restricted	2E alpha-dystroglycan	Expression is in Bergman glial fibers that pass through the molecular layer and external granule layer.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2E overlay , 2E alpha-dystroglycan	Expression is in Bergman glial fibers.
TS28: cerebellum internal granule cell layer	Present		2E alpha-dystroglycan , 2E overlay

Specimen not shown: postnatal day 32 (more

)

Structure	Level	Pattern	Note
TS28: cerebellum molecular layer	Present
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	Expression is in Bergman glial fibers.
TS28: cerebellum internal granule cell layer	Weak

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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