Antibody:	anti-nestin antisera 130
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	8A	Expression was predominantly confined to the columnar neuroepithelial cells, from the ventricular to the pial side.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Strong	Regionally restricted	8B	Expressed in the ventricular and subventricular zones. In the intermediate and marginal zones expression was largely confined to the elongated cell bodies of radial glial cells. Strong expression in the region immediately outside the marginal zone.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	8C	Expressed in the pial endfeet.

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Strong	Regionally restricted	8D	Expressed in the pial endfeet.