Dag1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82929 Henion TR, et al., Expression of dystroglycan, fukutin and POMGnT1 during mouse cerebellar development. Brain Res Mol Brain Res. 2003 Apr 10;112(1-2):177-81
Assay type:	RNA in situ
MGI Accession ID:	MGI:6324912
Gene symbol:	Dag1
Gene name:	dystroglycan 1

Probe:	dystroglycan cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1A: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebellum granule cell layer	Strong		1A	Expression is in proliferating granule cell precursors.
TS25: cerebellum layer	Strong	Regionally restricted	1A	Expression is in cells in deeper cerebellar layers that contain Bergmann glia, Purkinje cells, and other cell types.

Specimen 1D: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		1D
TS28: cerebellum internal granule cell layer	Strong		1D
TS28: cerebellum layer	Very strong	Single cells	1D	Expression is in Bergmann glia.
TS28: cerebellum Purkinje cell layer	Present		1D

Specimen 1G: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		1G
TS28: cerebellum internal granule cell layer	Strong		1G
TS28: cerebellum layer	Very strong	Single cells	1G	Expression is in Bergmann glia.
TS28: cerebellum Purkinje cell layer	Present		1G

Specimen 1J: postnatal day 32 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		1J	Expression is in Bergmann glial cells.
TS28: cerebellum internal granule cell layer	Present		1J

Specimen 3A: postnatal day 7 (more

)
Note: Double labeled: purple - Dag1; yellow - Gfap (assay MGI:6324928).

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3A , 3B	Expression is in Bergmann glial cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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