Probe:	Gas1 probe3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	1F	Expression in dorsal optic vesicle (future RPE domain).
TS15: eye mesenchyme	Present		1F	Expression in adjacent surface ectoderm and mesenchyme.
TS15: eye surface ectoderm	Present		1F	Expression in adjacent surface ectoderm and mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: optic cup	Present	Widespread	1G	Exrpession in entire optic cup.
TS17: optic cup outer layer	Strong	Regionally restricted	1G	High level of expression in presumptive retinal pigmented epithelium (RPE) in control eye.
TS17: eye surface ectoderm	Strong		1G
TS17: retina	Strong	Regionally restricted	1G	High levels of expression in presumptive RPE, and junction between neural retina and RPE.
TS17: neural retina	Present	Regionally restricted	1G	Expression in neural retina, with reduced level of expression in in central neural retina.
TS17: lens vesicle	Present	Regionally restricted	1G	Expression in lens and, with reduced levels of expression in posterior part of lens vesicle.v
TS17: eye mesenchyme	Present		1G

Structure	Level	Pattern	Image	Note
TS20: eye anterior segment	Present	Regionally restricted	1H	Expression in presumptive ciliary body, RPE and mesenchyme surrouding the eye.
TS20: pigmented retinal epithelium	Present		1H
TS20: eye mesenchyme	Present		1H	Expression in mesenchyme surrounding the eye.

Structure	Level	Pattern	Image	Note
TS22: eye anterior segment	Present	Regionally restricted	1I	Expression in presumptive ciliary body/iris
TS22: pigmented retinal epithelium	Present		1I
TS22: eye mesenchyme	Present		1I	Expression in mesenchyme surrounding eye (thought to give rise to cornea, eyelids and possibly sclera).