Gene Expression Data
Assay Details
Assay
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Reference:
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J:32017
Offield MF, et al., PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development. 1996 Mar;122(3):983-95
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6316656
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Gene symbol:
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Slc2a2
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Gene name:
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solute carrier family 2 (facilitated glucose transporter), member 2
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Results
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Specimen
6A:
embryonic day 18.5; Pdx1tm2Cvw/Pdx1 +
(more )
Note: Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
Specimen
6A:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cystic duct |
Strong |
Regionally restricted |
6A
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High levels of expression in epithelial lining of cystic duct.
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Specimen
6B:
embryonic day 18.5; Pdx1tm2Cvw/Pdx1 +
(more )
Note: Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
Specimen
6B:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: pancreatic duct |
Present |
Regionally restricted |
6B
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Authors report expression in villi on basolateral surfaces of columnar epithelium.
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Specimen
6C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1tm2Cvw
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: pancreatic duct |
Strong |
Regionally restricted |
6C
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Intense expression in dorsal ductule epithelium having a cuboidal morphology similar to that of the cystic duct. Reduced expression in numerous evaginations of ductules.
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TS26: duodenum epithelium |
Strong |
Regionally restricted |
6C
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Continuation of intense expression into aberrant cuboidal epihtelium of rostral-most duodenum.
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Specimen
6D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1tm2Cvw
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: duodenum epithelium |
Strong |
Regionally restricted |
6D
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More intense expression in abnormal duodenal epithelium than in the villi. Patches of reduced expression in cuboidal epithelium corresponding to regions of transition to normal columnar epithelium.
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Specimen
6E:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1tm2Cvw
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Glut2 (Slc2a2); blue - Ins1 (assay MGI:6316654 and Ins2 (assay MGI:6316655).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: pancreatic duct |
Present |
Regionally restricted |
6E
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Expression in evaginations of dorsal ductule epithelium.
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