Col4a4 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:30369 Enders GC, et al., Developmental changes in seminiferous tubule extracellular matrix components of the mouse testis: alpha 3(IV) collagen chain expressed at the initiation of spermatogenesis. Biol Reprod. 1995 Dec;53(6):1489-99
Assay type:	Northern blot
MGI Accession ID:	MGI:6316313
Gene symbol:	Col4a4
Gene name:	collagen, type IV, alpha 4

Probe:	alpha4(IV) probe4
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 1_alpha4

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	9.5 kb	5.5 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
1	P newborn	TS27: testis	Weak (a)	Weak (a)	10 µg; total RNA	ICR		Male
5	P5	TS28: testis	Present (b)	Present (b)	10 µg; total RNA	ICR		Male
10	P10	TS28: testis	Strong (c)	Strong (c)	10 µg; total RNA	ICR		Male
20	P20	TS28: testis	Present	Present	10 µg; total RNA	ICR		Male
30	P30	TS28: testis	Weak (d)	Weak (d)	10 µg; total RNA	ICR		Male
40	P40	TS28: testis	Weak	Weak	10 µg; total RNA	ICR		Male
A	P adult	TS28: testis	Weak	Weak	10 µg; total RNA	ICR		Male

Notes:
(a) Low levels of expression in newborns, comparable to adult levels.
(b) Rising expression at 5 days after birth.
(c) Expression peaking at 10 days after birth.
(d) Expression dramatically decreased to essentially adult levels by 30 days after birth.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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