Probe:	Oasis cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: bone	Weak	Regionally restricted		Expression is at sites of intramembraneous bone formation.
TS20: cranium	Weak	Regionally restricted		Expression is at sites of intramembraneous bone formation.
TS20: lower jaw primordium	Weak	Regionally restricted		Expression is at sites of intramembraneous bone formation.
TS20: mandible primordium	Weak	Regionally restricted		Expression is at sites of intramembraneous bone formation.
TS20: maxilla	Weak	Regionally restricted		Expression is at sites of intramembraneous bone formation.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	1e	Expression is in the bony periosteal cuff of the alveolar bone.
TS22: maxilla	Present	Regionally restricted	1e	Expression is in the bony periosteal cuff of the alveolar bone.

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	1f	Expression is in the bony periosteal cuff of the alveolar bone.
TS22: maxilla	Present	Regionally restricted	1f	Expression is in the bony periosteal cuff of the alveolar bone.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	1g	Expression is in the outer bony cortex of the ribs.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Strong	Regionally restricted	1h	Expression is in the outer bony cortex of the ribs and is absent in the hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: mandible	Present	Regionally restricted	2e	Expression is in the bony periosteal cuff and the internal bone trabeculae of the alveolar bone.
TS24: periosteum	Strong	Regionally restricted	2e	Expression is around the mandible.

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present		2f

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	2g	Expression is in the outer bony cortex of the ribs and is absent in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: rib	Present	Regionally restricted	2h	Expression is in the outer bony cortex of the ribs and is absent in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: rib cartilage	Strong	Regionally restricted	3a	Expression is proximal and is absent in the distal cartilagenous ribs.

Structure	Level	Pattern	Image	Note
TS24: submandibular gland	Present		3b

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present	Regionally restricted	3c , 3d	Expression is in the preodontoblast-odontoblast layers.
TS24: enamel organ	Absent		3d , 3c

Structure	Level	Pattern	Image	Note
TS26: lower jaw	Weak		4d	Expression is at the periosteum around the bone and in the cancellous bone of the alveolar bone.
TS26: periosteum	Weak		4d	Expression is at the periosteum around the bone and in the cancellous bone of the alveolar bone.

Structure	Level	Pattern	Image	Note
TS26: rib	Present	Regionally restricted	4e	Expression is in the outer bony cortex. Expression is in cells undergoing endochondral ossification and in the region of membraneous ossification.

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Strong		4f	Expression is stronger in the region where precursor cells differentiate into active odontoblasts.
TS26: enamel organ	Absent		4f

Structure	Level	Pattern	Image	Note
TS22: dental papilla	Present
TS22: limb cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS24: long bone	Present
TS24: pancreas	Weak

Structure	Level	Pattern	Image	Note
TS26: long bone	Weak

Structure	Level	Pattern	Image	Note
TS28: rib	Present			After birth expression is seen in the ribs and is absent in other bones.