Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45219
Meyer RA, et al., Developmental regulation and asymmetric expression of the gene encoding Cx43 gap junctions in the mouse limb bud. Dev Genet. 1997;21(4):290-300
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6314561
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Gene symbol:
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Gja1
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Gene name:
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gap junction protein, alpha 1
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Probe:
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p132
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.0
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud ectoderm |
Present |
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1A
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Expression is in the in the distal ectoderm of the right forelimb bud and is absent in the left forelimb bud.
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Specimen
1B:
embryonic day 9.0
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: forelimb bud ectoderm |
Present |
Regionally restricted |
1B
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Expression is in the in the distal ectoderm of the right forelimb bud and is absent in the left forelimb bud.
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Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1C
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Expression is in the in the distal ectoderm of the right forelimb bud.
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Specimen
1D:
embryonic day 9.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
1D
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Expression is in the in the distal ectoderm of the left forelimb bud.
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Specimen
1E:
embryonic day 10.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud ectoderm |
Present |
Regionally restricted |
1E
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Expression is in the in the distal ectoderm and is weaker in the right hindlimb bud.
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| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
1E
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Expression is in the distal-posterior limb bud.
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Specimen
1F:
embryonic day 10.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
1F
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Expression is in the in the distal ectoderm and is weaker in the right hindlimb bud.
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Specimen
1G:
embryonic day 10.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1H:
embryonic day 11.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1I:
embryonic day 11.5
(more )
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1J:
embryonic day 12.5
(more )
Specimen
1J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot epithelium |
Present |
Regionally restricted |
1J
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Expression is in the apical ectodermal ridge.
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| TS20: hand epithelium |
Present |
Regionally restricted |
1J
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Expression is in the apical ectodermal ridge.
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Specimen
1K:
embryonic day 12.5
(more )
Specimen
1K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: foot epithelium |
Present |
Regionally restricted |
1K
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Expression is in the apical ectodermal ridge.
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| TS20: hand epithelium |
Present |
Regionally restricted |
1K
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Expression is in the apical ectodermal ridge.
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Specimen
4A:
embryonic day 10.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
embryonic day 10.5; lgl/ lgl
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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lgl/lgl
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4C:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
4C
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
4D:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud ectoderm |
Present |
Regionally restricted |
4D
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
Table 1 CD1 E9.5:
embryonic day 9.5
(more )
Specimen
Table 1 CD1 E9.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
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Expression is in the distal limb ectoderm. In all cases (14) expression was first detected in the right limb bud, followed by expression in the same region of the left limb bud.
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Specimen
Table 1 CD1 E10.5:
embryonic day 10.5
(more )
Specimen
Table 1 CD1 E10.5:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Table 1 SWR E9.5:
embryonic day 9.5
(more )
Specimen
Table 1 SWR E9.5:
(close )
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Genetic Background:
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SWR
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
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Expression is in the distal limb ectoderm.
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Specimen
Table 1 SWR E10.5:
embryonic day 10.5
(more )
Specimen
Table 1 SWR E10.5:
(close )
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Genetic Background:
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SWR
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Table 1 C57BL6 E9.5:
embryonic day 9.5
(more )
Note: 18-27 somites stage embryo.
Specimen
Table 1 C57BL6 E9.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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18-27 somites stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
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Expression is in the distal limb ectoderm.
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Specimen
Table 1 C57BL6 E10.5:
embryonic day 10.5
(more )
Note: 25-31 somites stage embryo.
Specimen
Table 1 C57BL6 E10.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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25-31 somites stage embryo.
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Specimen
Table 1 iv/iv E10.5:
embryonic day 10.5; Dnah11iv/ Dnah11iv
(more )
Note: 28-29 somites stage embryo.
Specimen
Table 1 iv/iv E10.5:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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28-29 somites stage embryo.
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Specimen
Table 1 lgl/+ E10.5:
embryonic day 10.5; lgl/lgl +
(more )
Note: 29-35 somites stage embryo.
Specimen
Table 1 lgl/+ E10.5:
(close )
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Genetic Background:
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involves: C3H * C57BL/6
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Mutant Allele(s):
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lgl/lgl+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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29-35 somites stage embryo.
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