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Gene Expression Data
Assay Details
Assay
Reference: J:275336 Sulistomo HW, et al., Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction. J Biol Chem. 2019 Feb 22;294(8):2924-2934
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6314392
Gene symbol: Fhod3
Gene name: formin homology 2 domain containing 3
Reporter: lacZ
Direct Detection
Assay notes: Embryos were fixed in 1% formaldehyde, 0.4% glutaraldehyde; postfixed in 3.7% paraformaldehyde.
Results
Specimen 1C +/- upper: embryonic day 9.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present 1C +/- upper Expression in neural tube restricted to hindbrain.
TS15: future forebrain Absent 1C +/- upper
TS15: future midbrain Absent 1C +/- upper
Specimen 1C -/- upper: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present 1C -/- upper Expression in neural tube restricted to hindbrain.
TS15: future forebrain Absent 1C -/- upper
TS15: future midbrain Absent 1C -/- upper
Specimen 1C +/- lower: embryonic day 9.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present 1C +/- lower Expression in neural tube restricted to hindbrain.
TS15: future midbrain Absent 1C +/- lower
Specimen 1C -/- lower: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present 1C -/- lower Expression in neural tube restricted to hindbrain.
TS15: future midbrain Absent 1C -/- lower
Specimen 1D +/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 1D +/- Segmented pattern of expression in hindbrain. No expression in inter-rhombomere boundaries.
TS15: rhombomere 1 Present 1D +/- Expression only in rhombomere 1 to 6.
TS15: rhombomere 2 Present 1D +/- Expression only in rhombomere 1 to 6.
TS15: rhombomere 3 Present 1D +/- Expression only in rhombomere 1 to 6.
TS15: rhombomere 4 Present 1D +/- Expression only in rhombomere 1 to 6.
TS15: rhombomere 5 Present 1D +/- Expression only in rhombomere 1 to 6.
TS15: rhombomere 6 Present 1D +/- Expression only in rhombomere 1 to 6.
Specimen 1D -/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 1D -/- Segmented pattern of expression in hindbrain. No expression in inter-rhombomere boundaries.
TS15: rhombomere 1 Present 1D -/- Expression only in rhombomere 1 to 6;
TS15: rhombomere 2 Present 1D -/- Expression only in rhombomere 1 to 6;
TS15: rhombomere 3 Present 1D -/- Expression only in rhombomere 1 to 6;
TS15: rhombomere 4 Present 1D -/- Expression only in rhombomere 1 to 6;
TS15: rhombomere 5 Present 1D -/- Expression only in rhombomere 1 to 6;
TS15: rhombomere 6 Present 1D -/- Expression only in rhombomere 1 to 6;
Specimen 2A R4 +/-: embryonic day 8.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS13: rhombomere 4 Present Regionally restricted 2A R4 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS13: rhombomere 4 floor plate Absent 2A R4 +/-
Specimen 2A R4 -/-: embryonic day 8.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS13: rhombomere 4 Present Regionally restricted 2A R4 -/- Authors report pattern of expression was essentially same.
TS13: rhombomere 4 floor plate Absent 2A R4 -/- Authors report pattern of expression was essentially same.
Specimen 2A R5 +/-: embryonic day 8.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS13: rhombomere 5 Present 2A R5 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS13: rhombomere 5 floor plate Absent 2A R5 +/-
Specimen 2A R5 -/-: embryonic day 8.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS13: rhombomere 5 Present 2A R5 -/- Authors report pattern of expression was essentially same.
TS13: rhombomere 5 floor plate Absent 2A R5 -/- Authors report pattern of expression was essentially same.
Specimen 2B R4 +/-: embryonic day 9.0; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS14: rhombomere 4 lateral wall Present 2B R4 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS14: rhombomere 4 floor plate Absent 2B R4 +/-
TS14: rhombomere 4 roof plate Absent 2B R4 +/-
Specimen 2B R4 -/-: embryonic day 9.0; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS14: rhombomere 4 lateral wall Present 2B R4 -/- Authors report pattern of expression was essentially same.
TS14: rhombomere 4 floor plate Absent 2B R4 -/-
TS14: rhombomere 4 roof plate Absent 2B R4 -/-
Specimen 2B R5 +/-: embryonic day 9.0; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS14: rhombomere 5 lateral wall Present 2B R5 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS14: rhombomere 5 floor plate Absent 2B R5 +/-
TS14: rhombomere 5 roof plate Absent 2B R5 +/-
Specimen 2B R5 -/-: embryonic day 9.0; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS14: rhombomere 5 lateral wall Present 2B R5 -/- Authors report pattern of expression was essentially same.
TS14: rhombomere 5 floor plate Absent 2B R5 -/- Authors report pattern of expression was essentially same.
TS14: rhombomere 5 roof plate Absent 2B R5 -/- Authors report pattern of expression was essentially same.
Specimen 2C R4 +/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present 2C R4 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS15: rhombomere 4 floor plate Absent 2C R4 +/-
TS15: rhombomere 4 roof plate Absent 2C R4 +/-
Specimen 2C R4 -/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 lateral wall Present 2C R4 -/- Authors report pattern of expression was essentially same.
TS15: rhombomere 4 floor plate Absent 2C R4 -/- Authors report pattern of expression was essentially same.
TS15: rhombomere 4 roof plate Absent 2C R4 -/- Authors report pattern of expression was essentially same.
Specimen 2C R5 +/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3+ (more )
Structure Level Pattern Image Note
TS15: rhombomere 5 lateral wall Present 2C R5 +/- Robust expression in closing neural plate. Expression restricted to the lateral plate.
TS15: rhombomere 5 floor plate Absent 2C R5 +/-
TS15: rhombomere 5 roof plate Absent 2C R5 +/-
Specimen 2C R5 -/-: embryonic day 9.5; Fhod3tm1Hsum/Fhod3tm1Hsum (more )
Structure Level Pattern Image Note
TS15: rhombomere 5 lateral wall Present 2C R5 -/- Authors report pattern of expression was essentially same.
TS15: rhombomere 5 floor plate Absent 2C R5 -/- Authors report pattern of expression was essentially same.
TS15: rhombomere 5 roof plate Absent 2C R5 -/- Authors report pattern of expression was essentially same.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory