Gene Expression Data
Assay Details
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Assay
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Reference:
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J:246772
Gkatzis K, et al., Interaction Between ALK1 Signaling and Connexin40 in the Development of Arteriovenous Malformations. Arterioscler Thromb Vasc Biol. 2016 Apr;36(4):707-17
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6314150
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Gene symbol:
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Gja5
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Gene name:
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gap junction protein, alpha 5
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Reporter:
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GFP
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Direct Detection
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Results
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Specimen
2D Gja5EGFP/+:
postnatal day 7; Gja5tm1Lumi/Gja5 +
(more  )
Note: Triple labeled: green - Gja5 reporter; blue - Isolectin B4; red - Acta2 (assay MGI:6314128).
Specimen
2D Gja5EGFP/+:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gja5tm1Lumi/Gja5+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Gja5 reporter; blue - Isolectin B4; red - Acta2 (assay MGI:6314128).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina blood vessel |
Present |
Regionally restricted |
2D Gja5EGFP/+ right
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2D Gja5EGFP/+ left
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Expression is in the arteries.
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Specimen
2D Acvrl1+/-;Gja5EGFP/+:
postnatal day 7; Acvrl1tm2.1Spo/Acvrl1 +, Gja5tm1Lumi/Gja5 +
(more )
Note: Triple labeled: green - Gja5 reporter; blue - Isolectin B4; red - Acta2 (assay MGI:6314128).
Specimen
2D Acvrl1+/-;Gja5EGFP/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Acvrl1tm2.1Spo/Acvrl1+
Gja5tm1Lumi/Gja5+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Gja5 reporter; blue - Isolectin B4; red - Acta2 (assay MGI:6314128).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina blood vessel |
Present |
Regionally restricted |
2D Acvrl1+/-;GjaEGFP/+ left
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2D Acvrl1+/-;GjaEGFP/+ right
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The area of arterial expression has expanded in this mutant.
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Specimen
S5A Gja5EGFP/+:
embryonic day 12.5; Gja5tm1Lumi/Gja5 +
(more )
Note: Double labeled: green - Gja5 reporter; red - Pecam1 (assay MGI:6314155).
Specimen
S5A Gja5EGFP/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gja5 reporter; red - Pecam1 (assay MGI:6314155).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: arterial blood vessel |
Present |
Regionally restricted |
S5A Gja5EGFP/+
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Expression is in the mesencephalic artery.
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Specimen
S5A Acvrl1+/-;Gja5EGFP/+:
embryonic day 12.5; Acvrl1tm2.1Spo/Acvrl1 +, Gja5tm1Lumi/Gja5 +
(more )
Note: Double labeled: green - Gja5 reporter; red - Pecam1 (assay MGI:6314155).
Specimen
S5A Acvrl1+/-;Gja5EGFP/+:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Acvrl1tm2.1Spo/Acvrl1+
Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Gja5 reporter; red - Pecam1 (assay MGI:6314155).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: arterial blood vessel |
Present |
Regionally restricted |
S5A Acvrl1+/-;Gja5EGFP/+
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Expression in the mesencephalic artery is enlarged in this mutant.
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Specimen
S5D Gja5EGFP/+ lung:
embryonic day 17.5; Gja5tm1Lumi/Gja5 +
(more )
Specimen
S5D Gja5EGFP/+ lung:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S5D Acvrl1+/-;Gja5EGFP/+ lung:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Acvrl1tm2.1Spo/Acvrl1+
Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: arterial blood vessel |
Present |
Regionally restricted |
S5D Acvrl1+/-;Gja5EGFP/+ lung
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Expression in the arteries is enlarged in this mutant.
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| TS25: lung vascular element |
Present |
Regionally restricted |
S5D Acvrl1+/-;Gja5EGFP/+ lung
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Expression in the arteries is enlarged in this mutant.
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Specimen
S5D Gja5EGFP/+ intestine:
embryonic day 17.5; Gja5tm1Lumi/Gja5 +
(more )
Specimen
S5D Gja5EGFP/+ intestine:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: arterial blood vessel |
Present |
Regionally restricted |
S5D Gja5EGFP/+ intestine
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Expression in the intestinal arteries.
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Specimen
S5D Acvrl1+/-;Gja5EGFP/+ intestine:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Acvrl1tm2.1Spo/Acvrl1+
Gja5tm1Lumi/Gja5+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: arterial blood vessel |
Present |
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S5D Acvrl1+/-;Gja5EGFP/+ intestine
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Expression in the intestinal arteries is enlarged in this mutant.
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Analysis Tools