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Gene Expression Data
Assay Details
Assay
Reference: J:246772 Gkatzis K, et al., Interaction Between ALK1 Signaling and Connexin40 in the Development of Arteriovenous Malformations. Arterioscler Thromb Vasc Biol. 2016 Apr;36(4):707-17
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314128
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody
Results
Specimen 2D Control: postnatal day 7 (more )
Note: Triple labeled: red - Acta2; blue - Isolectin B4; green - Gja5 reporter (assay MGI:6314150).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present Regionally restricted 2D Control Expression is in vascular smooth muscle cells.
TS28: retina blood vessel Present Regionally restricted 2D Control Expression is in vascular smooth muscle cells.
Specimen 2D Gja5EGFP/+: postnatal day 7; Gja5tm1Lumi/Gja5+ (more )
Note: Triple labeled: red - Acta2; blue - Isolectin B4; green - Gja5 reporter (assay MGI:6314150).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present Regionally restricted 2D Gja5EGFP/+ left Expression is in vascular smooth muscle cells.
TS28: retina blood vessel Present Regionally restricted 2D Gja5EGFP/+ left Expression is in vascular smooth muscle cells.
Specimen 2D Acvrl1+/-: postnatal day 7; Acvrl1tm2.1Spo/Acvrl1+ (more )
Note: Triple labeled: red - Acta2; blue - Isolectin B4; green - Gja5 reporter (assay MGI:6314150).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present Regionally restricted 2D Acvrl1+/- Expression is in vascular smooth muscle cells.
TS28: retina blood vessel Present Regionally restricted 2D Acvrl1+/- Expression is in vascular smooth muscle cells.
Specimen 2D Acvrl1+/-;Gja5EGFP/+: postnatal day 7; Acvrl1tm2.1Spo/Acvrl1+, Gja5tm1Lumi/Gja5+ (more )
Note: Triple labeled: red - Acta2; blue - Isolectin B4; green - Gja5 reporter (assay MGI:6314150).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Strong Regionally restricted 2D Acvrl1+/-;GjaEGFP/+ left Expression in vascular smooth muscle cells is expanded in this mutant.
TS28: retina blood vessel Strong Regionally restricted 2D Acvrl1+/-;GjaEGFP/+ left Expression in vascular smooth muscle cells is expanded in this mutant.
Specimen 4A Control: postnatal day 60-90 (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4A Control
Specimen 4A Gja5EGFP/+: postnatal day 60-90; Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4A Gja5EGFP/+
Specimen 4A Acvrl+/-: postnatal day 60-90; Acvrl1tm2.1Spo/Acvrl1+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4A Acvrl+/-
Specimen 4A Acvrl1+/-;Gja5EGFP/+: postnatal day 60-90; Acvrl1tm2.1Spo/Acvrl1+, Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4A Acvrl1+/-;Gja5EGFP/+ In this mutant the main arteries are enlarged.
Specimen 4G Control: postnatal week 8 (more )
Note: Double labeled: green - Acta2; red - dihydroethidium.
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4G Control
Specimen 4G Gja5EGFP/+: postnatal week 8; Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - dihydroethidium.
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4G Gja5EGFP/+
Specimen 4G Acvrl+/-: postnatal week 8; Acvrl1tm2.1Spo/Acvrl1+ (more )
Note: Double labeled: green - Acta2; red - dihydroethidium.
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4G Acvrl+/-
Specimen 4G Acvrl1+/-;Gja5EGFP/+: postnatal week 8; Acvrl1tm2.1Spo/Acvrl1+, Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - dihydroethidium.
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 4G Acvrl1+/-;Gja5EGFP/+
Specimen 5A Control: postnatal day 60-90 (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 5A Control
Specimen 5A Acvrl+/-: postnatal day 60-90; Acvrl1tm2.1Spo/Acvrl1+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 5A Acvrl+/-
Specimen 5A Gja5EGFP/+: postnatal day 60-90; Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 5A Gja5EGFP/+
Specimen 5A Acvrl1+/-;Gja5EGFP/+: postnatal day 60-90; Acvrl1tm2.1Spo/Acvrl1+, Gja5tm1Lumi/Gja5+ (more )
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:6314155).
Structure Level Pattern Image Note
TS28: blood vessel smooth muscle Present 5A Acvrl1+/-;Gja5EGFP/+
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory