Antibody:	anti-CRABP1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS16: atrium cardiac muscle	Present		S1a	Evenly distributed.
TS16: primitive ventricle cardiac muscle	Present		S1a	Including the myocardium of the outflow tract. Evenly distributed.
TS16: outflow tract mesenchyme	Present		S1a	Expressed in the myocardium.
TS16: visceral pericardium	Weak		S1a

Structure	Level	Pattern	Image	Note
TS18: atrium cardiac muscle	Present		S2a	Evenly distributed.
TS18: outflow tract cardiac muscle	Present	Regionally restricted	S2a	Evenly distributed.
TS18: primitive ventricle cardiac muscle	Present		S2a	Evenly distributed.
TS18: visceral pericardium	Weak		S2a

Structure	Level	Pattern	Image	Note
TS18: atrium cardiac muscle	Present		S2b	Evenly distributed.
TS18: outflow tract cardiac muscle	Present		S2b	Evenly distributed.
TS18: primitive ventricle cardiac muscle	Present		S2b	Evenly distributed.
TS18: visceral pericardium	Weak		S2b

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		S3a	Evenly distributed.
TS20: outflow tract cardiac muscle	Present		S3a	Evenly distributed.
TS20: ventricle cardiac muscle	Present		S3a	Evenly distributed.
TS20: endocardial cushion	Present	Single cells	S3a	Expressed in some cells.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		S3b	Evenly distributed.
TS20: outflow tract cardiac muscle	Present		S3b	Evenly distributed.
TS20: ventricle cardiac muscle	Present		S3b	Evenly distributed.

Structure	Level	Pattern	Image	Note
TS21: outflow tract endocardial cushion	Present	Regionally restricted	S4a	Expressed in some of the mesenchyme cells.
TS21: arch of aorta	Present	Uniform	S4a	Homogenous staining.
TS21: atrium cardiac muscle	Present	Uniform	S4a	Homogenous staining.
TS21: outflow tract cardiac muscle	Present	Uniform	S4a	Homogenous staining.
TS21: pulmonary artery	Present	Uniform	S4a	Homogenous staining.
TS21: ventricle cardiac muscle	Present	Uniform	S4a	Homogenous staining.

Structure	Level	Pattern	Image	Note
TS21: outflow tract cardiac muscle	Present		S4b
TS21: outflow tract endocardial cushion	Present	Regionally restricted	S4b	Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: heart	Present		S7a
TS22: visceral pericardium	Weak		S7a

Structure	Level	Pattern	Image	Note
TS25: cardiac muscle tissue	Present	Uniform
TS25: outflow tract	Present	Regionally restricted		Expressed in the mesenchymal cells of the aorto-pulmonary septum.
TS25: great vessel of heart	Absent
TS25: semilunar valve	Absent
TS25: atrioventricular valve	Present	Regionally restricted		Expressed in the mesenchyme, localized close to the surface adjacent to the ventricular lumen.
TS25: visceral pericardium	Weak

Structure	Level	Pattern	Image	Note
TS20: visceral pericardium	Weak

Structure	Level	Pattern	Image	Note
TS21: aortico-pulmonary spiral septum	Present	Regionally restricted		Expressed in some of the mesenchymal cells.
TS21: visceral pericardium	Weak

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Present	Uniform		Homogenous staining.
TS22: atrium cardiac muscle	Present	Uniform		Homogenous staining.
TS22: pulmonary artery	Present	Uniform		Homogenous staining.
TS22: ventricle cardiac muscle	Present	Uniform		Homogenous staining.
TS22: endocardial cushion	Present	Regionally restricted		Expressed in some of the mesenchymal cells of the outflow tract cushions.
TS22: aortico-pulmonary spiral septum	Present	Regionally restricted		Expressed in some of the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: outflow tract	Present	Regionally restricted		Expressed in the mesenchymal cells of the aorto-pulmonary septum.
TS23: cardiac muscle tissue	Present	Uniform		Evenly distributed.
TS23: great vessel of heart	Absent
TS23: semilunar valve	Absent
TS23: atrioventricular valve	Present	Regionally restricted		Expressed in the mesenchyme, localized close to the surface adjacent to the ventricular lumen.
TS23: visceral pericardium	Weak