Antibody:	anti-NCAM [H28.123]
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS22: integumental system mesenchyme	Moderate	Regionally restricted	1D	Expression was detected in a thin layer of mesenchymal cells just below the dermal-epithelial junction.
TS22: dermis	Present	Regionally restricted	1D	Staining revealed many axonal sections in the dermis which reached close to the dermal-epithelial junction.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present	Regionally restricted	2D	When the placodal cells began to move down into the dermis to form a hair plug, the follicle epithelium stained positively for Ncam1.
TS24: integumental system mesenchyme	Present	Regionally restricted	2D	Expression was detected in the layer of mesenchyme just below the dermal-epithelial junction.

Structure	Level	Pattern	Image	Note
TS24: hair epidermal placode	Absent		2G	The awl placodes were usually negative for Ncam1 staining. One sample with premature stratification exhibited positively stained placodal cells.

Structure	Level	Pattern	Image	Note
TS25: hair follicle	Strong	Regionally restricted	3D	The lateral follicle epithelium of most hair plugs stained intensely. The staining pattern of awl hair plugs was virtually identical to wild type.
TS25: dermis	Present	Regionally restricted	3D	Expression was restricted to the interfollicular mesenchyme in the dermis.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Strong	Regionally restricted	4D	Intense expression was detected in the upper caudal epithelium of elongating follicles.
TS26: hair follicle dermal papilla	Present		4D
TS26: hair root sheath	Present	Regionally restricted	4D	Expression was detected in the mesenchymal sheath.
TS26: integumental system mesenchyme	Present	Regionally restricted	4D	Expression was detected in interfollicular mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present		5D	Expression was detected in and around a normal hair plug.

Structure	Level	Pattern	Image	Note
TS25: hair follicle	Present	Regionally restricted	6E	The abnormal tylotrich follicles showed intense staining in the upper caudal follicle epithelium.

Structure	Level	Pattern	Image	Note
TS25: hair follicle dermal papilla	Present		6G	Expression was detected in the dermal papilla, as in wild type.
TS25: hair root sheath	Present	Regionally restricted	6G	Expression was detected in the mesenchymal sheath, as in wild type.

Structure	Level	Pattern	Image	Note
TS25: hair follicle	Moderate	Regionally restricted	6H	Expression was detected in follicle epithelium. Unlike in wild type, staining was found in awl hair plugs.
TS25: integumental system mesenchyme	Moderate	Regionally restricted	6H	Moderate staining was detected in the mesenchyme surrounding the follicle.
TS25: epidermis	Present	Regionally restricted	6H	The basal epidermal layer was stained, unlike in wild type.

Structure	Level	Pattern	Image	Note
TS26: hair follicle	Present		7E	Normal staining pattern was noted in the morphologically abnormal follicles.

Structure	Level	Pattern	Image	Note
TS26: dermis	Present	Regionally restricted	7G	The dermal mesenchyme stained more intensely than in wild type skin.