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Gene Expression Data
Assay Details
Assay
Reference: J:216140 Hanson MG, et al., Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. Dev Biol. 2014 Nov 1;395(1):84-95
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6304275
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament-M
Detection system: Alexa Fluor 568
Results
Specimen 2G: embryonic day 18.5 (more )
Note: Double labeled: red - Nefm; green - Tubb3 (assay MGI:6304276).
Structure Level Pattern Image Note
TS26: spinal cord cervical region Present Regionally restricted 2G Expression in axons.
Specimen 2G inset: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: spinal cord cervical region Present Regionally restricted 2G inset Expression in axons.
Specimen 2H: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Double labeled: red - Nefm; green - Tubb3 (assay MGI:6304276).
Structure Level Pattern Image Note
TS26: spinal cord cervical region Present Regionally restricted 2H Authors report no significant difference at the level of the cervical enlargment compared to littermate controls.
Specimen 2H inset: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Structure Level Pattern Image Note
TS26: spinal cord cervical region Present Regionally restricted 2H inset Authors report no significant difference at the level of the cervical enlargment compared to littermate controls.
Specimen 2J: embryonic day 18.5 (more )
Note: Double labeled: red - Nefm; green - Tubb3 (assay MGI:6304276).
Structure Level Pattern Image Note
TS26: spinal cord lumbar region Present Regionally restricted 2J Expression in axons.
Specimen 2J inset: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: spinal cord lumbar region Present Regionally restricted 2J inset Expression in axons.
Specimen 2K: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Double labeled: red - Nefm; green - Tubb3 (assay MGI:6304276).
Structure Level Pattern Image Note
TS26: spinal cord lumbar region Weak Regionally restricted 2K Authores report a significant decrease in expression compared to littermate controls.
Specimen 2K inset: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Structure Level Pattern Image Note
TS26: spinal cord lumbar region Weak Regionally restricted 2K inset Authores report a significant decrease in expression compared to littermate controls.
Specimen 3C/F: embryonic day 18.5 (more )
Note: Triple labeled: red - Nefm; green - S100b (assay MGI:6304278); blue - Hoechst.
Structure Level Pattern Image Note
TS26: sciatic nerve Present Widespread 3C , 3F Expression in axons throughout section.
Specimen 3G/J: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm; green - S100b (assay MGI:6304278); blue - Hoechst.
Structure Level Pattern Image Note
TS26: sciatic nerve Weak Regionally restricted 3G , 3J Expression in cells around perimeter. Greatly reduced expression in axons in central region.
Specimen 3K/N: embryonic day 15.5 (more )
Note: Triple labeled: red - Nefm; green - S100b (assay MGI:6304279); blue - Hoechst.
Structure Level Pattern Image Note
TS23: sciatic nerve Present Regionally restricted 3K , 3N Expression in axons.
Specimen 3O/R: embryonic day 15.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm; green - S100b (assay MGI:6304279); blue - Hoechst.
Structure Level Pattern Image Note
TS23: sciatic nerve Present Regionally restricted 3R , 3O Authors report similar levels and distribution of expression in axons.
Specimen 4A/D: embryonic day 18.5 (more )
Note: Triple labeled: red - Nefm; green - Sv2a (assay MGI:6304280); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: soleus Present Regionally restricted 4D , 4A Authors report stereotypical pattern of expression in axons.
Specimen 4E/H: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm; green - Sv2a (assay MGI:6304280); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: soleus Present Regionally restricted 4H , 4E Authors report decreased co-localization with Sv2a and alpha-BTX.
Specimen 5A: embryonic day 15.5 (more )
Note: Triple labeled: red - Nefm and Sv2a (assay MGI:6304281); blue - alpha-BTX.
Structure Level Pattern Image Note
TS23: diaphragm Present Regionally restricted 5A Expression in phrenic axons.
Specimen 5B: embryonic day 15.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm and Sv2a (assay MGI:6304281); blue - alpha-BTX.
Structure Level Pattern Image Note
TS23: diaphragm Present Regionally restricted 5B Authors report many axons extend abnormally beyond AChR clusters.
Specimen 5D: embryonic day 18.5 (more )
Note: Triple labeled: red - Nefm and Sv2a (assay MGI:6304281); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5D Expression in phrenic axons.
Specimen 5E: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm and Sv2a (assay MGI:6304281); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5E Authors report many axons extend abnormally beyond AChR clusters.
Specimen 5G: embryonic day 18.5 (more )
Note: Triple labeled: red - Nefm; green - Sox10 (assay MGI:6304282); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5G Expression in phrenic axons.
Specimen 5H: embryonic day 18.5; Pex10m1Nisw/Pex10m1Nisw (more )
Note: Triple labeled: red - Nefm; green - Sox10 (assay MGI:6304282); blue - alpha-BTX.
Structure Level Pattern Image Note
TS26: diaphragm Present Regionally restricted 5H Authors report many axons extend abnormally beyond AChR clusters.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory