Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47986
Dellovade TL, et al., The gonadotropin-releasing hormone system does not develop in Small-Eye (Sey) mouse phenotype. Brain Res Dev Brain Res. 1998 May 15;107(2):233-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6303885
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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Anti-N-CAM
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to other - see notes
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Assay notes:
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The secondary antibody was labeled with Vector SG chromogen.
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Results
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Specimen
not shown:
embryonic day 13.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
1A:
embryonic day 14.5
(more )
Note: Double labeled: blue/gray - Ncam1; red/brown - Gnrh1 (assay MGI:6303851).
Specimen
1A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: blue/gray - Ncam1; red/brown - Gnrh1 (assay MGI:6303851).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: olfactory epithelium |
Present |
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1A
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| TS22: nasal septum |
Present |
Regionally restricted |
1B
,
1A
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Expression was detected in cords migrating along the nasal septum.
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| TS22: forebrain |
Present |
Regionally restricted |
1A
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Immunoreactive fibers were seen entering the developing forebrain.
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Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forebrain |
Present |
Regionally restricted |
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Immunoreactive fibers were entering the forebrain.
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Specimen
1C:
embryonic day 16.5
(more )
Note: Double labeled: blue/gray - Ncam1; red/brown - Gnrh1 (assay MGI:6303851).
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: blue/gray - Ncam1; red/brown - Gnrh1 (assay MGI:6303851).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: forebrain |
Present |
Regionally restricted |
1C
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Immunoreactive fibers were entering the forebrain.
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Specimen
1D:
embryonic day 18.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: nose |
Absent |
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| TS21: cranial nerve |
Present |
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Immunoreactivity in cranial nerve fibers appeared normal in the Sey/Sey embryos.
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| TS21: neocortex |
Weak |
Regionally restricted |
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Ncam1 immunoreactivity in the frontal cortex appeared less intense.
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: nose |
Absent |
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| TS23: cranial nerve |
Present |
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Immunoreactivity in cranial nerve fibers appeared normal in the Sey/Sey embryos.
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| TS23: neocortex |
Present |
Regionally restricted |
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Ncam1 immunoreactivity in the frontal cortex appeared less intense.
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Specimen
not shown:
embryonic day 13.5; Pax6Sey/Pax6 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: neocortex |
Present |
Regionally restricted |
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Expression was detected in the frontal cortex.
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Specimen
not shown:
embryonic day 15.5; Pax6Sey/Pax6 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: neocortex |
Present |
Regionally restricted |
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Expression was detected in the frontal cortex.
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Analysis Tools