Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48563
Sporle R, et al., Paradox segmentation along inter- and intrasomitic borderlines is followed by dysmorphology of the axial skeleton in the open brain (opb) mouse mutant. Dev Genet. 1998;22(4):359-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6296001
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 10.5
(more  )
Note: TS17
Specimen
5A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS17
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5A
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Expression marked the continuous, elongating myotome layers that were clearly segmented from their epaxial to their hypaxial extremities.
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Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6 * NMRI
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Mutant Allele(s):
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Rab23opb/Rab23opb
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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TS17
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: myotome |
Present |
Regionally restricted |
5B
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Expression in most myotomes bridged the somite borders and the thoracic hypaxial myotomes were delayed and exhibited a tendency of bifurcation. Expression also bridged the somite borders between the truncated epaxial myotomes of the cervical-thoracic transition.
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Analysis Tools
