Probe:	Emx2 probe17
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted		Expression only in anterior dorsal part of neural tube, fated to become telencephalic cortical regions.

Structure	Level	Pattern	Image	Note
TS13: nephrogenic cord rostral part	Present	Regionally restricted	1A	Earliest expression in urogenital system in pronephros during turning of embryo.

Structure	Level	Pattern	Image	Note
TS14: nephrogenic cord rostral part	Strong	Regionally restricted	1B	After complete turning, intense expression bilaterally in caudal part of trunk region at level of pronephros.
TS14: prosencephalon	Present		1B	Expression in the forebrain.

Structure	Level	Pattern	Image	Note
TS14: nephric duct, pronephric portion	Present	Regionally restricted	1C	Expression in epithelium of not-yet-canalized pronephric ducts.

Structure	Level	Pattern	Image	Note
TS16: nephric duct	Strong	Regionally restricted	1D	Strong expression in epithelial cells of Wolffian duct.
TS16: mesonephros	Present	Regionally restricted	1D	Expression in early epithelial mesonephric cells.

Structure	Level	Pattern	Image	Note
TS16: nephric duct	Strong	Regionally restricted	1E	Intense expression in Wolffian duct.

Structure	Level	Pattern	Image	Note
TS21: mesonephric tubule	Present		1F
TS21: ovary	Present		1F

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Present	Regionally restricted	1G	Expression in kidneys localized in branches of ureteric bud.

Structure	Level	Pattern	Image	Note
TS21: primitive collecting duct	Strong	Regionally restricted	1H	Intense expression in repeatedly branching ureteric bud.

Structure	Level	Pattern	Image	Note
TS16: mesonephros	Present		2A
TS16: urogenital ridge	Present	Regionally restricted	2A	Expression in coelomic epithelium covering primordia of genital ridges.

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Strong	Regionally restricted	2B	Expression on medial aspect of urogenital ridges.
TS19: mesonephros	Present		2B
TS19: nephric duct	Present		2B

Structure	Level	Pattern	Image	Note
TS21: nephric duct of female	Present		2C	Expression in Mullerian ducts, Wollfian ducts and ovary.
TS21: ovary	Present		2C	Expression in Mullerian ducts, Wollfian ducts and ovary.
TS21: paramesonephric duct of female	Present		2C	Expression in Mullerian ducts, Wollfian ducts and ovary.

Structure	Level	Pattern	Image	Note
TS21: nephric duct	Present		2D	Expression in Mullerian and Wollfian ducts.
TS21: paramesonephric duct	Present		2D	Expression in Mullerian and Wollfian ducts.

Structure	Level	Pattern	Image	Note
TS21: mesonephric tubule	Present		2E	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: nephric duct of male	Present		2E	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: paramesonephric duct of male	Present		2E	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: testis	Weak		2E	Faint expression in testis.

Structure	Level	Pattern	Image	Note
TS21: mesonephric tubule	Present		2F	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: nephric duct of female	Present		2F	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: paramesonephric duct of female	Present		2F	Expression in Mullerian and Wollfian ducts with mesonephric tubules.
TS21: ovary	Strong		2F	Intense expression in ovary.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		3A	Authors report comparable level of expression at level of "indifferent" gonads of male and female embryos.
TS19: mesonephric tubule	Strong		3A	Strong expression in epithelial cells of mesonephric tubule, Wolffian duct.
TS19: nephric duct	Strong		3A	Strong expression in epithelial cells of mesonephric tubule, Wolffian duct.
TS19: ureteric bud	Strong	Regionally restricted	3A	Strong expression in ureteric bud invading metanephrogenic mesenchyme.
TS19: metanephric mesenchyme	Absent		3A	No expression in metanephrogenic mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		3B	Authors report comparable level of expression at level of "indifferent" gonads of male and female embryos.
TS19: mesonephric tubule	Strong		3B	Strong expression in epithelial cells of mesonephric tubule, Wolffian duct.
TS19: nephric duct	Strong		3B	Strong expression in epithelial cells of mesonephric tubule, Wolffian duct.
TS19: ureteric bud	Strong	Regionally restricted	3B	Strong expression in ureteric bud in the context of metanephrogenic mesenchyme.
TS19: metanephric mesenchyme	Absent		3B	No expression in metanephrogenic mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present			Authors report same levels of expression in gonads of male and female embryos.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present			Authors report same levels of expression in gonads of male and female embryos.

Structure	Level	Pattern	Image	Note
TS21: testis	Present	Regionally restricted	3C	Authors report "striped" appearance of expression in testis.
TS21: nephric duct of male	Present		3C	Expression in Wolffian and Mullerian ducts.
TS21: paramesonephric duct of male	Present		3C	Expression in Wolffian and Mullerian ducts.

Structure	Level	Pattern	Image	Note
TS21: ovary	Strong		3D
TS21: nephric duct of female	Present		3D	Expression in Wolffian and Mullerian ducts.
TS21: paramesonephric duct of female	Present		3D	Expression in Wolffian and Mullerian ducts.

Structure	Level	Pattern	Image	Note
TS21: testis	Trace	Regionally restricted	3E	Very faint expression in testis, almost exclusively localized in peripheral part of gonad.

Structure	Level	Pattern	Image	Note
TS21: ovary	Strong	Diffuse	3G