Probe:	gelatinase 92kDa probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Multiple probes were described. It is possible gelatinase 92kDa probe2 (MGI:6295376) was used for some of the specimens in this assay.

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent
TS12: yolk sac endoderm	Strong	Regionally restricted		High levels of expression localized to endoderm component of yolk sac.
TS12: trophoblast giant cell	Strong	Regionally restricted		High expression in trophoblast giant cells invading maternal tissues.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent
TS13: yolk sac endoderm	Present	Regionally restricted		High levels of expression localized to endoderm component of yolk sac.
TS13: trophoblast giant cell	Present	Regionally restricted		High expression in trophoblast giant cells invading maternal tissues.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent
TS15: yolk sac endoderm	Present	Regionally restricted		High levels of expression localized to endoderm component of yolk sac.
TS15: trophoblast giant cell	Present	Regionally restricted		High expression in trophoblast giant cells invading maternal tissues.

Structure	Level	Pattern	Image	Note
TS19: 4th ventricle	Present	Regionally restricted	1A	Expression in germinal zone cells lining lateral ventricle, fourth venticle, and optic recess of diencephalon.
TS19: lateral ventricle	Present	Regionally restricted	1A	Expression in germinal zone cells lining lateral ventricle, fourth venticle, and optic recess of diencephalon.
TS19: optic recess	Present	Regionally restricted	1A	Expression in germinal zone cells lining lateral ventricle, fourth venticle, and optic recess of diencephalon.
TS19: Rathke's pouch	Present	Regionally restricted	1A	Exression in epithelium of olfactory (nasal) pit and Rathke's pouch.
TS19: nasal pit	Present	Regionally restricted	1A	Exression in epithelium of olfactory (nasal) pit and Rathke's pouch.
TS19: 1st branchial arch	Present	Regionally restricted	1A	Expression in mandibular component of first branchial arch.

Structure	Level	Pattern	Image	Note
TS21: brain ventricle	Strong			Increased expression in brain vesicles, spinal cord, choroid plexus, cerebellum, and optic area
TS21: cerebellum	Strong			Increased expression in brain vesicles, spinal cord, choroid plexus, cerebellum, and optic area
TS21: choroid plexus	Strong			Increased expression in brain vesicles, spinal cord, choroid plexus, cerebellum, and optic area
TS21: eye	Strong			Increased expression in brain vesicles, spinal cord, choroid plexus, cerebellum, and optic area
TS21: spinal cord	Strong			Increased expression in brain vesicles, spinal cord, choroid plexus, cerebellum, and optic area

Structure	Level	Pattern	Image	Note
TS23: lateral ventricle	Strong	Regionally restricted	2A	Highest expression localized to lining of lateral ventricle along choroidal fissure, extending to hippocampal and basal ganglia and septal areas.
TS23: cortical plate	Strong		2A	Strong expression in neocortical neuroepithelial cells, and in cells composing neocortex or cortical plate.
TS23: neocortex	Strong		2A	Strong expression in neocortical neuroepithelial cells, and in cells composing neocortex or cortical plate.
TS23: cerebral cortex intermediate zone	Weak		2A	Much weaker expression in intermediate zone.
TS23: lateral ventricle choroid plexus	Present		2A

Structure	Level	Pattern	Image	Note
TS23: 4th ventricle choroid plexus	Strong		3A	High levels of expression in modified ependymal cells of choroid plexus of fourth ventricle, as well as mesenchymal cells in area of developing vasculature.
TS23: cerebellum	Strong	Regionally restricted	3A	High levels of expression localized to cerebellar neuroepithelium and cerebellar vermis.
TS23: cerebellar peduncle	Strong		3A
TS23: rhombic lip	Strong		3A

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Strong	Regionally restricted	3C	High expression in cells composing cerebellar vermis.

Structure	Level	Pattern	Image	Note
TS23: spinal cord mantle layer	Strong		4A
TS23: dorsal grey horn	Very strong		4A	Highest intensity of expression in dorsal horns.
TS23: spinal cord marginal layer	Strong	Single cells	4A	High expression in sporadic cells at marginal layer.
TS23: dorsal root ganglion	Present		4A

Structure	Level	Pattern	Image	Note
TS23: spinal cord gray matter	Present	Widespread	4C	Expression in virtually all cells forming gray matter.
TS23: spinal cord white matter	Present	Regionally restricted	4C	Expression in some cells in developing white matter.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5A	Expression was distributed in neurons forming ganglion cell layer of retina.
TS23: lens capsule	Present	Regionally restricted	5A	Expression was detected in the cuboidal epithelium of the lens capsule.
TS23: lens fiber	Present	Regionally restricted	5A	Expression in proliferating epithelial cells forming lens fibers.
TS23: optic tract	Present	Regionally restricted	5A	Expression was distributed in cell bodies of optic tract.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5C	Expression in ganglion cell layer of retina.
TS23: pigmented retinal epithelium	Absent		5C

Structure	Level	Pattern	Image	Note
TS23: lens	Present	Regionally restricted	5E	Expression localized to membranous component of zonula fibers.