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Gene Expression Data
Assay Details
Assay
Reference: J:266589 Cohen-Tayar Y, et al., Pax6 regulation of Sox9 in the mouse retinal pigmented epithelium controls its timely differentiation and choroid vasculature development. Development. 2018 Aug 9;145(15):dev163691
Assay type: RNA in situ
MGI Accession ID: MGI:6293896
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Probe: Sox9 probe37
Probe preparation: labelled with Cy3 DNA
Assay notes: Single molecule fluorescence in situ hybridization (sm-FISH)
Results
Specimen 1C Proximal Control: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Double labeled: red - Sox9; green - Pax6 (assay MGI:6293898).
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C Control Proximal There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
Specimen 1C Proximal Pax6 cKO: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C cKO Proximal Sox9 expression level was significantly elevated in Pax6 cKO. The central to peripheral gradient was maintained for Sox9 transcripts.
Specimen 1C Middle Control: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Double labeled: red - Sox9; green - Pax6 (assay MGI:6293898).
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C Control Middle There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
Specimen 1C Middle Pax6 cKO: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C cKO Middle Sox9 expression level was significantly elevated in Pax6 cKO. The central to peripheral gradient was maintained for Sox9 transcripts.
Specimen 1C Distal Control: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Double labeled: red - Sox9; green - Pax6 (assay MGI:6293898).
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C Control Distal There was a spatial dose-dependent inverse relationship between Pax6 and Sox9, with a significant negative correlation between the mRNA levels. A central to peripheral gradient was noted for Sox9 transcripts.
Specimen 1C Distal Pax6 cKO: embryonic day 13.5; Pax6tm2Pgr/Pax6tm2Pgr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: pigmented retinal epithelium Present 1C cKO Distal Sox9 expression level was significantly elevated in Pax6 cKO. The central to peripheral gradient was maintained for Sox9 transcripts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory