Pax6 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:266589 Cohen-Tayar Y, et al., Pax6 regulation of Sox9 in the mouse retinal pigmented epithelium controls its timely differentiation and choroid vasculature development. Development. 2018 Aug 9;145(15):dev163691
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6293892
Gene symbol:	Pax6
Gene name:	paired box 6

Antibody:	Anti-Pax6 (AD2.38 sc-32766)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1A ©

Specimen 1A: embryonic day 15.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	Present		1A

2A ©

Specimen 2A: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		2A

2B ©

Specimen 2B: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm, Tg(Dct-cre)1Apdn/0 (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		2B	No difference was noted in Pax6 expression level in the Sox9 cKO.

S1B ©

S1F ©

Specimen S1F: embryonic day 13.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present	Widespread	S1F , S1B	In the early optic cup, Pax6 was detected throughout the retinal pigmented epithelium.

S1C ©

S1G ©

Specimen S1G: embryonic day 15.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	Present		S1C , S1G

S1D ©

S1H ©

Specimen S1H: embryonic day 19.5 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS26: pigmented retinal epithelium	Ambiguous		S1D , S1H

S1E ©

S1I ©

Specimen S1I: postnatal day 8 (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Ambiguous		S1E , S1I	Pax6 was gradually replaced by Sox9 in the retinal pigmented epithelium.

S2A ©

S2A' ©

S2B ©

S2B' ©

Specimen S2A/B: embryonic day 13.5; Pax6^tm2Pgr/Pax6^tm2Pgr (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S2B , S2A' , S2A , S2B'

S2D ©

S2D' ©

S2E ©

S2E' ©

Specimen S2D/E: embryonic day 13.5; Pax6^tm2Pgr/Pax6^tm2Pgr (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Absent		S2D , S2D' , S2E , S2E'

S4A ©

S4A' ©

S4B ©

S4B' ©

Specimen S4A/B: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm (more

)
Note: Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S4B' , S4A , S4B , S4A'

S4D ©

S4D' ©

S4E ©

S4E' ©

Specimen S4D/E: embryonic day 13.5; Sox9^tm2Crm/Sox9^tm2Crm, Tg(Dct-cre)1Apdn/0 (more

)
Note: Conditional mutant. Double labeled: green - Pax6; red - Sox9 (assay MGI:6293891).

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		S4D' , S4E' , S4E , S4D	No difference was noted in Pax6 expression level in the Sox9 cKO.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24