Gene Expression Data
Assay Details
Assay
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Reference:
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J:85764
Annicotte JS, et al., Pancreatic-duodenal homeobox 1 regulates expression of liver receptor homolog 1 during pancreas development. Mol Cell Biol. 2003 Oct;23(19):6713-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6281987
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Gene symbol:
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Nr5a2
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Gene name:
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nuclear receptor subfamily 5, group A, member 2
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Probe:
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LRH-1 cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1B e7.5:
embryonic day 7.5
(more )
Specimen
1B e7.5:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 7.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1B e9.5:
embryonic day 9.5
(more )
Specimen
1B e9.5:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
1B e13.5:
embryonic day 13.5
(more )
Specimen
1B e13.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
e13.5 not shown:
embryonic day 13.5
(more )
Specimen
e13.5 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1B e16.5:
embryonic day 16.5
(more )
Specimen
1B e16.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
6C transversal +/+:
embryonic day 9.5
(more )
Specimen
6C transversal +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
6C transversal -/-:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Pdx1tm1Ted/Pdx1tm1Ted
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: hepatic primordium |
Weak |
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6C transversal PDX-1-/-
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Authors report significantly stronger expression in wild type relative to mutant tissues
|
TS15: pancreas primordium |
Weak |
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6C transversal PDX-1-/-
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Authors report significantly stronger expression in wild type relative to mutant tissues
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Specimen
6C sagittal +/+:
embryonic day 9.5
(more )
Specimen
6C sagittal +/+:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6C sagittal -/-:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
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Mutant Allele(s):
|
Pdx1tm1Ted/Pdx1tm1Ted
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: hepatic primordium |
Weak |
|
6C sagittal PDX-1-/-
|
Authors report significantly stronger expression in wild type relative to mutant tissues
|
TS15: pancreas primordium |
Weak |
|
6C sagittal PDX-1-/-
|
Authors report significantly stronger expression in wild type relative to mutant tissues
|
|