Antibody:	anti-calretinin
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present		1A'' , 1A'	Expression was in Cajal-Retzius cells.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		1B'' , 1B'	Large numbers of Cajal-Retzius cells were ectopically located in deeper cortical areas.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present		1C'' , 1C	Expression was in Cajal-Retzius cells.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		1D , 1D''	Ectopic Cajal-Retzius cells were double stained with Calb2 and Reln.
TS23: cerebral cortex marginal layer	Present		1D , 1D''	Normally placed Cajal-Retzius cells were double stained with Calb2 and Reln.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	4A , 4A''	Expression was detected in Cajal-Retzius cells in a thin layer near the pia.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	4B , 4B''	Expression was detected in Cajal-Retzius cells. Using a cre-line specifically expressed in hem-derived Cajal-Retzius cells, there was no obvious change in Cajal-Retzius cell location.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present	Regionally restricted	5A , 5A''	Expression was detected in a tight layer of Cajal-Retzius cells near the pial surface.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present	Regionally restricted	5B , 5B''	Expression was detected in a tight layer of Cajal-Retzius cells near the pial surface in most cortical areas in the mutant. Only occasional areas showed Cajal-Retzius cell displacement.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex marginal layer	Present	Regionally restricted	8A	Expression was detected in Cajal-Retzius cells near the pia.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		8B , 8b , 8a	Expression was detected in Cajal-Retzius cells. In the mutant, large numbers of Cajal-Retzius cells were in deeper regions of the cortical wall. In areas devoid of radial glia, the Cajal-Retzius cells were dramatically displaced. In areas with more radial glia, the Cajal-Retzius cells retained their position near the pia.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Present	Regionally restricted	S1A' , S1A	Expression was detected in Cajal-Retzius cells near the pia.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		S1B , S1B'	Expression was detected in Cajal-Retzius cells. In the mutant, large numbers of Cajal-Retzius cells were displaced in deeper regions of the cortex.